Detailed information of TA system TA166576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5135341..5136599
• Replicon: chromosome
• Accession: NZ_CP058331
• Organism: Pseudomonas aeruginosa strain ACR22

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: HWQ50_RS23810
• Protein ID: WP_003112528.1
• Coordinates: 5135341..5136096 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HWQ50_RS23815
• Protein ID: WP_003086329.1
• Coordinates: 5136231..5136599 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ50_RS23800	5130512..5133055	+	2544	WP_023110293.1	acyl-homoserine-lactone acylase QuiP	-
HWQ50_RS23805	5133211..5134572	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HWQ50_RS23810	5135341..5136096	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
HWQ50_RS23815	5136231..5136599	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HWQ50_RS23820	5136672..5137958	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
HWQ50_RS23825	5138014..5139603	-	1590	WP_079280589.1	aldehyde dehydrogenase family protein	-
HWQ50_RS23830	5139682..5140158	+	477	WP_003112531.1	hypothetical protein	-
HWQ50_RS23835	5140161..5140436	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T166576 WP_003112528.1 NZ_CP058331:c5136096-5135341 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T166576 NZ_CP074354:3863007-3863159 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT166576 WP_003086329.1 NZ_CP058331:c5136599-5136231 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT166576 NZ_CP074354:c3862958-3862901 [Escherichia coli]
GTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7