Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 88966..89609 | Replicon | plasmid pIT-1825-FIIK1 |
| Accession | NZ_CP058327 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain LC-1825/18 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | GLO21_RS27900 | Protein ID | WP_001044770.1 |
| Coordinates | 88966..89382 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | GLO21_RS27905 | Protein ID | WP_001261282.1 |
| Coordinates | 89379..89609 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GLO21_RS27865 (GLO21_027850) | 84508..84744 | - | 237 | WP_000993386.1 | broad-spectrum mercury transporter MerE | - |
| GLO21_RS27870 (GLO21_027855) | 84741..85103 | - | 363 | WP_001277456.1 | mercury resistance co-regulator MerD | - |
| GLO21_RS27875 (GLO21_027860) | 85121..86815 | - | 1695 | WP_000105636.1 | mercury(II) reductase | - |
| GLO21_RS27880 (GLO21_027865) | 86867..87289 | - | 423 | WP_001340589.1 | organomercurial transporter MerC | - |
| GLO21_RS27885 (GLO21_027870) | 87325..87435 | - | 111 | Protein_94 | mercuric transport protein periplasmic component | - |
| GLO21_RS27895 (GLO21_027880) | 88203..88892 | - | 690 | Protein_96 | ATP-binding protein | - |
| GLO21_RS27900 (GLO21_027885) | 88966..89382 | - | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| GLO21_RS27905 (GLO21_027890) | 89379..89609 | - | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| GLO21_RS27910 (GLO21_027895) | 89566..90027 | + | 462 | WP_072093212.1 | hypothetical protein | - |
| GLO21_RS27915 (GLO21_027905) | 90514..90822 | + | 309 | WP_004152336.1 | hypothetical protein | - |
| GLO21_RS27920 (GLO21_027910) | 90850..91179 | + | 330 | WP_004152337.1 | hypothetical protein | - |
| GLO21_RS27925 (GLO21_027915) | 91205..91603 | + | 399 | WP_004171440.1 | hypothetical protein | - |
| GLO21_RS27930 (GLO21_027920) | 91610..91942 | + | 333 | WP_004152339.1 | hypothetical protein | - |
| GLO21_RS27935 (GLO21_027925) | 91942..92724 | + | 783 | WP_004152340.1 | site-specific integrase | - |
| GLO21_RS27940 (GLO21_027930) | 93616..93846 | - | 231 | WP_011977773.1 | hypothetical protein | - |
| GLO21_RS27945 (GLO21_027935) | 93938..94411 | - | 474 | WP_004152341.1 | YkgJ family cysteine cluster protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-Ib / blaOXA-9 / blaKPC-40 / blaTEM-1A | - | 1..120302 | 120302 | |
| - | flank | IS/Tn | - | - | 94531..95799 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T166572 WP_001044770.1 NZ_CP058327:c89382-88966 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T166572 NZ_CP074354:c2366170-2365994 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGTGTCACCCTAGCGATGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGGTTTGAGTTAA
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGTGTCACCCTAGCGATGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGGTTTGAGTTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT166572 WP_001261282.1 NZ_CP058327:c89609-89379 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT166572 NZ_CP074354:c2365948-2365532 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |