Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5263201..5263826 Replicon chromosome
Accession NZ_CP058325
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-1825/18

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GLO21_RS25975 Protein ID WP_002882817.1
Coordinates 5263201..5263584 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GLO21_RS25980 Protein ID WP_004150355.1
Coordinates 5263584..5263826 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GLO21_RS25960 (GLO21_025950) 5260567..5261469 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GLO21_RS25965 (GLO21_025955) 5261466..5262101 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GLO21_RS25970 (GLO21_025960) 5262098..5263027 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GLO21_RS25975 (GLO21_025965) 5263201..5263584 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GLO21_RS25980 (GLO21_025970) 5263584..5263826 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
GLO21_RS25985 (GLO21_025975) 5264031..5264948 + 918 WP_002882812.1 alpha/beta hydrolase -
GLO21_RS25990 (GLO21_025980) 5264962..5265903 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GLO21_RS25995 (GLO21_025985) 5265948..5266385 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
GLO21_RS26000 (GLO21_025990) 5266382..5267242 - 861 WP_002882807.1 virulence factor BrkB family protein -
GLO21_RS26005 (GLO21_025995) 5267236..5267835 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T166570 WP_002882817.1 NZ_CP058325:c5263584-5263201 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T166570 NZ_CP074354:1966445-1966548 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT166570 WP_004150355.1 NZ_CP058325:c5263826-5263584 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT166570 NZ_CP074354:c1966586-1966315 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References