Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1227125..1227744 Replicon chromosome
Accession NZ_CP058325
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-1825/18

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GLO21_RS05900 Protein ID WP_002892050.1
Coordinates 1227125..1227343 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GLO21_RS05905 Protein ID WP_002892066.1
Coordinates 1227370..1227744 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GLO21_RS05865 (GLO21_005865) 1223172..1223435 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
GLO21_RS05870 (GLO21_005870) 1223435..1223575 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GLO21_RS05875 (GLO21_005875) 1223572..1224270 - 699 WP_002892021.1 GNAT family protein -
GLO21_RS05880 (GLO21_005880) 1224371..1225822 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
GLO21_RS05885 (GLO21_005885) 1225797..1226267 - 471 WP_002892026.1 YlaC family protein -
GLO21_RS05890 (GLO21_005890) 1226288..1226428 + 141 WP_004147370.1 hypothetical protein -
GLO21_RS05895 (GLO21_005895) 1226400..1226966 - 567 WP_002892030.1 maltose O-acetyltransferase -
GLO21_RS05900 (GLO21_005900) 1227125..1227343 - 219 WP_002892050.1 HHA domain-containing protein Toxin
GLO21_RS05905 (GLO21_005905) 1227370..1227744 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GLO21_RS05910 (GLO21_005910) 1228230..1231376 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GLO21_RS05915 (GLO21_005915) 1231399..1232592 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166556 WP_002892050.1 NZ_CP058325:c1227343-1227125 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T166556 NZ_CP074352:c141924-141613 [Siccibacter colletis]
ATGGTGAATGAAAAATGGACGCCCCATACGCGCCTTCACGTAGTGAAACAACTGGTGCTGGACGGCAAGGTTCGTACTAC
CGGCAGCGCGCTACGCCATGCGCTCGCCGCAGGCTATCGCGAGCCGGCACTACAGCAGATGTGTGACGTCATTTTGCGTC
TGCAGCCCGATGACTTTTATAAAAGCATGACGACACTGGCCGATCACAGGATTTGGCAGGACGTCTGGCATGGTCATGAC
GGTGACACAGCGCTTTATATCAAACTGACCGTCATAGACGATCTGTTAATCGTCTCTTATAAGGAGCTATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT166556 WP_002892066.1 NZ_CP058325:c1227744-1227370 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT166556 NZ_CP074352:c141612-141211 [Siccibacter colletis]
ATGAAATGTCCGGTCTGCGGCGGTGCAGAACTCATTCATGACACCCGCGATATTCCCTGGGTCTATAAAGGCCAGCAAAG
CCTGTTGCCCGCTATTACCGGTGACTATTGTCCTTGTTGCGGGGAAATCATCCTCAATGAAGAACAAAGCAACCGCTATG
CACGTGCTATCCGCGAAGCGAAAAAAGCGATAAATGCCCGGCACATCGATCCGGCATTTATCGCCCGGATACGGCAAAAA
ATGGCGCTGGACCAGCGAGAAGCAGCCGCGCTTTTCGGTGGTGGGGCTAATGCATTCTCACGCTATGAAACCGGCAAAAC
GCAGCCGCCACTGGCAATGATTAAGCTCTTCAAGCTGCTGGATAAGCACCCCGAACTGATGGCGGAAGTCAAAACGCTGT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References