Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 612161..612971 Replicon chromosome
Accession NZ_CP058325
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-1825/18

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag GLO21_RS02935 Protein ID WP_002887280.1
Coordinates 612438..612971 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GLO21_RS02930 Protein ID WP_002887278.1
Coordinates 612161..612427 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GLO21_RS02910 (GLO21_002905) 608033..608377 - 345 WP_002887266.1 cation efflux system protein CusF -
GLO21_RS02915 (GLO21_002910) 608395..609780 - 1386 WP_002887267.1 efflux transporter outer membrane subunit -
GLO21_RS02920 (GLO21_002915) 609952..610635 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
GLO21_RS02925 (GLO21_002920) 610625..612058 + 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
GLO21_RS02930 (GLO21_002925) 612161..612427 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
GLO21_RS02935 (GLO21_002930) 612438..612971 + 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
GLO21_RS02940 (GLO21_002935) 613019..614140 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T166555 WP_002887280.1 NZ_CP058325:612438-612971 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T166555 NZ_CP074352:80849-81217 [Siccibacter colletis]
ATGGCACCAGGGATGAAATTTCCGCATAAGGGAGAAATCTGGCACGTAAATGGCGACCCGACAAAAGGCCGTGAATTTCA
GGGTCCACACTATTATCTGGTGATTACCGACAAACAGCTGGTTAAAGCATTGGGCGTCGCCACCTGCGTGCCCATTACCA
CAGGTGGAAGCGCCGCGCGATCTGCACAGGTTACGGTCATGCTGGACGGCGGCAGTACCGATAAAGGCAGCGTAACCGGT
GTGGCCCTGTGTTACATGCTGAGAAGTATGGATCTCAAAGCACGTGGTGCCCGGTATCACTCCACAGTGGAAGCCGCCGT
CATGCGTGAGATACTTGATAAGGTTGTTGATCTTATCGACCCACAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT166555 WP_002887278.1 NZ_CP058325:612161-612427 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT166555 NZ_CP074352:81363-81629 [Siccibacter colletis]
ATGGCGCGTACCATGACCGTCGATCTGGGTGAAGAGCTTCGTGGTTTTGTGGAGTCTCTGATCGCTACCGGAGACTACCG
AACGCAAAGCGAAGTGATTCGGGACTCGCTGCGTTTACTGCGAGAACAGCAGGCGCAGTCGCATCTGCAGCAGTTGCGAG
CGCTCATCAATGAAGGGGTGAACAGCGGTGCCCCGCAAGAGTGGAAGCAGGATGCTTTCCTTCAAAACCTAAAAGCCAGG
GATCGGCATGATGACGAGACGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References