Detailed information of TA system TA166544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 704328..704875
• Replicon: chromosome
• Accession: NZ_CP058321
• Organism: Halomonas sp. SH5A2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HXW73_RS03405
• Protein ID: WP_186254902.1
• Coordinates: 704573..704875 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U7FV42
• Locus tag: HXW73_RS03400
• Protein ID: WP_022990189.1
• Coordinates: 704328..704585 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXW73_RS03360	699376..699807	+	432	WP_186254893.1	helix-turn-helix domain-containing protein	-
HXW73_RS03365	699940..700062	+	123	WP_186254895.1	SDR family NAD(P)-dependent oxidoreductase	-
HXW73_RS03370	700093..701663	-	1571	Protein_647	IS3 family transposase	-
HXW73_RS03375	701761..702390	+	630	WP_186254896.1	SDR family oxidoreductase	-
HXW73_RS03380	702432..703127	+	696	WP_186254897.1	type 1 glutamine amidotransferase	-
HXW73_RS03385	703206..703442	-	237	WP_186254898.1	thioredoxin family protein	-
HXW73_RS03390	703562..703903	+	342	WP_186254899.1	helix-turn-helix transcriptional regulator	-
HXW73_RS03395	703950..704141	+	192	WP_186254901.1	hypothetical protein	-
HXW73_RS03400	704328..704585	+	258	WP_022990189.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HXW73_RS03405	704573..704875	+	303	WP_186254902.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HXW73_RS03410	705044..705439	+	396	WP_186254903.1	GNAT family N-acetyltransferase	-
HXW73_RS03415	705436..706398	+	963	WP_186254904.1	ribonuclease Z	-
HXW73_RS03420	706562..707215	+	654	WP_186254905.1	DsbA family oxidoreductase	-
HXW73_RS03425	707315..708511	-	1197	WP_186254906.1	homoserine O-acetyltransferase	-
HXW73_RS03430	708570..709739	-	1170	WP_186255896.1	UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	691517..704875	13358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11617.25 Da
• Isoelectric Point: 4.3640

>T166544 WP_186254902.1 NZ_CP058321:704573-704875 [Halomonas sp. SH5A2]
MAEVIWTEPALQELDAIAEYIALDNPAAASRLVEEIFDKTDRLEDFPHSGRIPPEFSNSVYREVVVPPCRIFYREAEKQV
LVLYVMREERQLRAYMLGNS

Nucleotide

• Length: 303 bp

>T166544 NZ_CP074350:692671-693627 [Pantoea dispersa]
ATGGATGATGTCATAGCGTTATTTCCTGATGTAGCCGATGCATTAGGCATCGAATCTGTCGCTATCGTGGAAAAGGATTT
CTATATTGTTGAATTGCTGCGGCTGTTACAGCCTTTAAGCTTCGGGAGTCATCAGCTGGTATTTTGTGGTGGAACCGCTT
TATCTAAGGCGGGTATTGCGCTTAACCGCATGTCTGAAGATGTGGATATCAAGCTTGTTCCCGTCGCCGATTTCTTTGCT
AAAAACTCACGCACGCAGCGCAAAGCCATTCGTAAACACATCATCGCCGAAATAACGGAAACCTTAGCCGCTTCAGCATT
TTTTCGTTTTGATGAGGCACACCCTAAAACTATTCGCGATGAGTATCGTTATAGTGAACTTCCCATCCGTTATCCGCAGA
AATACGCTCAGGTGCCATGCCTGAGGCCCTTCATTAAGCTGGAGATCATTGAGGCGACGTTACTTGAACCGCCAGAAAAG
CGTGATATTCAGTCGCTTGTCTCTGCCCTGACACAACAGGGCGTTACGGTGCCGGATTTTCCCTGCGCAACAGTGCTCAC
TACCCAGGCGGAAAAGCTGGTGTCCATGATGCGCCGCACTGCGGCATACATCAGAAGGCCGCAACGGGAAGACGATGCAT
CTTTAGTACGGCATGTGTACGATAATTTTTGCATTGCCAGCACCCATGAGATTGATTTAGCACGGCTACAAGCGTATGTG
CGTCAGTGTATTAAAGAGGATATTGAGCGGTATGGTATTCAATATCCCGAATTCTGCACCTCCCCAATTGCAGAACTCAG
AATGGGTTTAGAAGAGCTGGCAGAGAATCCTGTTCACCGCCAGCGTTATCAGCAATTCGTTGTGCCCATGGTTTACGGCC
AGTCTCCGGCTTCCTGGGAGGAGGCATTTGCCCGTTTTCGGCAGACGGCTATCGAGGTCCTCAGCGCCGCACGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9887.41 Da
• Isoelectric Point: 8.5042

>AT166544 WP_022990189.1 NZ_CP058321:704328-704585 [Halomonas sp. SH5A2]
MKVELVTNLKRQATKILADLHLSKEPVLITEHGQPSAYLVDVQDYEFMQRRLELLEGLSRGERAVLEGRTYSQSEAREKM
SKWLK

Nucleotide

• Length: 258 bp

>AT166544 NZ_CP074350:692283-692678 [Pantoea dispersa]
ATGACTATTAAAGCGCGCATGCAGTCACGGCTGAAACGCTCTAAGCGTTATGTTTTCACGCGTGATGACTTCAACGATCT
TGCCGGTTACGACCAGATTGGCCGTGTACTGCGGGAACTGGTGAACGAAGGTCAGTTGCTTAAGGTCGGCTATGGTGTGT
ACACCAAAGCAAGAAAGAACGCGATTACGGGCAAGGTAATGCCTGCTGCACCCGGCGGTTCTGCTGCCGTTGTCATCGAG
ACGCTCGAACGTCTGAAGGTTGATTATCGTCTGGTTGGCGCGTCGGATGCCTATAACAGTGGGAAAACCACGCAGATTCC
TGCATCCATGCAGATCAAAACGTCGCCACGTTTTAAACGCACCTTGTCAGTAGGAAGCAGTAAATTAAATGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A1I1I6C4