Detailed information of TA system TA166529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2260138..2260667
• Replicon: chromosome
• Accession: NZ_CP058312
• Organism: Staphylococcus aureus strain BLR-DV

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HW113_RS11350
• Protein ID: WP_000621175.1
• Coordinates: 2260138..2260500 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HW113_RS11355
• Protein ID: WP_000948331.1
• Coordinates: 2260497..2260667 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW113_RS11330	2257117..2257887	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
HW113_RS11335	2257862..2258341	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
HW113_RS11340	2258343..2258669	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HW113_RS11345	2258788..2259789	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HW113_RS11350	2260138..2260500	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HW113_RS11355	2260497..2260667	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HW113_RS11360	2260752..2261900	-	1149	WP_001281145.1	alanine racemase	-
HW113_RS11365	2261966..2262325	-	360	WP_000581200.1	holo-ACP synthase	-
HW113_RS11370	2262329..2262820	-	492	WP_001205910.1	PH domain-containing protein	-
HW113_RS11375	2262807..2264390	-	1584	WP_001294626.1	PH domain-containing protein	-
HW113_RS11380	2264383..2264862	-	480	WP_001287088.1	hypothetical protein	-
HW113_RS11385	2265070..2265630	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T166529 WP_000621175.1 NZ_CP058312:c2260500-2260138 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T166529 NZ_CP074346:c1295988-1295737 [Pasteurella atlantica]
ATGCAAGTAATGACCTATACTGAAGCTCGTCAAAATCTAGCACAAACAATGACAGCAACAGCAGAAAATTGTGAGCCAGT
GCTTATTACAAGAAGTAACAATGAAAGTTGTGTTCTTATTTCTTTACGTGAATATGAAGCATTAGAAGAAACAGCCTATC
TTTTACGCTCACCAGCAAATGCTAAGCATTTATTGGAAAGTATTGAAGAGCTAAAATTAGGTAATGTTACTAAAAGAGAA
TTGATTGAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT166529 WP_000948331.1 NZ_CP058312:c2260667-2260497 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT166529 NZ_CP074346:c1295740-1295486 [Pasteurella atlantica]
ATGATTGTCTGTTTTACTGACAAAGGTTGGGAAGATTATCTTTATTGGCAAGAGACTGATAAAAAAACCTTAAAACGAAT
TAATTCTTTGATAAAAGAAATCAAGCGAAGCCCTTTTATAGGTAAAGGGAAGCCAGAACCATTAAAACATTCTTTAAGTG
GTTTTTGGTCAAGACGAATAACGGAGGAACATAGATTGGTTTATCGGGTATCTGAGGATGCTTTATCTATTGTTGCCTGT
CGCTATCACTATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1