Detailed information of TA system TA166525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 970148..970924
• Replicon: chromosome
• Accession: NZ_CP058312
• Organism: Staphylococcus aureus strain BLR-DV

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: HW113_RS05005
• Protein ID: WP_000031108.1
• Coordinates: 970772..970924 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: HW113_RS05000
• Protein ID: WP_001251224.1
• Coordinates: 970148..970747 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW113_RS04980	966064..967521	+	1458	WP_000649907.1	ABC transporter permease subunit	-
HW113_RS04985	967514..968236	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
HW113_RS04990	968387..969514	+	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
HW113_RS04995	969519..969989	+	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
HW113_RS05000	970148..970747	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
HW113_RS05005	970772..970924	+	153	WP_000031108.1	hypothetical protein	Toxin
HW113_RS05010	971701..972096	+	396	WP_000901021.1	hypothetical protein	-
HW113_RS05015	972292..973677	+	1386	WP_000116224.1	class II fumarate hydratase	-
HW113_RS05020	974140..974961	-	822	WP_112366563.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T166525 WP_000031108.1 NZ_CP058312:970772-970924 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T166525 NZ_CP074233:c3409129-3408788 [Salmonella bongori CFSAN000510]
ATGCAAACCCAATACAACCACCCACACCGGGCGACCCCGTCACAGCCGTCGCCTGTCGAAATCTGGCAAAAACTGCTGAC
CCATTTACTGGCTAAACATTATGGGCTCGAACTCAGTGACACGCCGTTTAGCGTAGAGAAGGTGATTCAGGAACACATTG
ACGCCGGTATCACGCTGGCTAATGCCGTGAACTTTATTGTAGAGAAATACGAGCTGGTTCGTATCGATCGCAAGGGATTT
AGCTGGCAGGAACAATCACCCTATCTTCGGGCTGTAGACATTCTGCGGGCGCGACAGGCTACGGGTCTGTTGCGACGTCA
ACGCTATCTCGCGGCACACTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT166525 WP_001251224.1 NZ_CP058312:970148-970747 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT166525 NZ_CP074233:c3409514-3409173 [Salmonella bongori CFSAN000510]
ATGGACACAAATGAAACACAATCCACGCCAGTATGGGGACTACGGAACGATGTCTTCCCGTCATTTGGCGCCCGGTTAGT
TCAGGAAGGTAATCACCTGCACTATCTGGCTGACCGGGCGGGTATTTACGGTGAATTTACCCCGGCGCAACTAAAGACTC
TGAATATCGTATTTCCAATGTTTATTAAACGCATGGAGGTCGCTTTACGCACTGGCGCACTCAATCCGCGCGAAGCCAGA
CGCTTTACGACTGCACTCCGGGGCATAATCTGCGAAGCCGACACTCGCGGATCGTTCGGTTACGTTTATCTTTCGCTTTA
CCCAACCGTCGTTAAAAACTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4