Detailed information of TA system TA166521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 93042..94665
• Replicon: plasmid pAMPD03
• Accession: NZ_CP058311
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HXS78_RS24995
• Protein ID: WP_095858479.1
• Coordinates: 93042..94367 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: HXS78_RS25000
• Protein ID: WP_024136327.1
• Coordinates: 94372..94665 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS24965	89307..89687	+	381	WP_001426317.1	hypothetical protein	-
HXS78_RS24970	89745..90410	+	666	WP_000490638.1	hypothetical protein	-
HXS78_RS24975	90454..91151	-	698	Protein_98	IS1 family transposase	-
HXS78_RS24980	91435..92250	-	816	WP_053276223.1	HNH endonuclease	-
HXS78_RS24985	92263..92673	-	411	WP_000985911.1	hypothetical protein	-
HXS78_RS24990	92775..92981	-	207	WP_000134171.1	hypothetical protein	-
HXS78_RS24995	93042..94367	-	1326	WP_095858479.1	type II toxin-antitoxin system HipA family toxin	Toxin
HXS78_RS25000	94372..94665	-	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
HXS78_RS25005	95269..95661	-	393	WP_000341066.1	hypothetical protein	-
HXS78_RS25010	96034..96375	+	342	WP_001151575.1	hypothetical protein	-
HXS78_RS25015	96467..96658	+	192	WP_000398480.1	hypothetical protein	-
HXS78_RS25020	96708..96989	+	282	WP_000077926.1	hypothetical protein	-
HXS78_RS25025	97552..98793	-	1242	WP_001053338.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-1.1 / aadA5 / oqxB / oqxA	-	1..217263	217263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50221.14 Da
• Isoelectric Point: 6.1927

>T166521 WP_095858479.1 NZ_CP058311:c94367-93042 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQAWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T166521 NZ_CP074233:828071-828484 [Salmonella bongori CFSAN000510]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTAATTTTATTGATGCCCTGGCCGTTGAGTTATACCCCATTGTGGATGATGCTACTGTCGCTGGTGGTGTTTGACTGCG
TTCGTAGTCAGCGCCGGATTAATACCTGTCAGGGAGAGATCAAGCTGCTAATGGATGGGCGCTTACGCTGGCAGGGGGAA
GACTGGGTGCTCCTGCGCCCGCCCTGGTTACTGAACAGCGGGATGGTGTTGCGTTTACGCGCCGAGTCCGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAGGCGGAATGGCGTGAGTTACGCCGTGTCATGTTGCAACAACCACTAT
CGGGCCAACACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT166521 WP_024136327.1 NZ_CP058311:c94665-94372 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT166521 NZ_CP074233:827824-828090 [Salmonella bongori CFSAN000510]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGCGACGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAGCTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1