Detailed information of TA system TA166516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 21029..21654
• Replicon: plasmid pAMPD02
• Accession: NZ_CP058310
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HXS78_RS23935
• Protein ID: WP_000911313.1
• Coordinates: 21256..21654 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: HXS78_RS23930
• Protein ID: WP_000450520.1
• Coordinates: 21029..21256 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS23930	21029..21256	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HXS78_RS23935	21256..21654	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HXS78_RS23940	21663..23816	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
HXS78_RS23945	24069..24800	-	732	WP_023565183.1	complement resistance protein TraT	-
HXS78_RS23950	24832..25329	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..129018	129018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T166516 WP_000911313.1 NZ_CP058310:21256-21654 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T166516 NZ_CP074200:851140-851433 [Halomonas sp. FeN2]
ATGACCGTTCCTAATGTCACTTTCAGTCACTACGATACTGCTGATTATCTTCAAACTGAGGAAGATATTGCGGCCTACCT
TGATGCAGTCATGGAAGAAAATGATCCTGCTCTTCTTGCCGCCGCACTTGGTGATATCGCCCGTGCCCGCAACATGAGCC
AACTCGCCAAAGACGTTGGAATGAGCCGGGAAGGGCTCTATAAAGCTCTTTCGGGGGAAGGCAATCCTGCATTTTCGACC
ATCAGCAAGGTCGCTAACTCCTTAGGGTTGCGCCTATCTATTAAACCGCTATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT166516 WP_000450520.1 NZ_CP058310:21029-21256 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT166516 NZ_CP074200:850839-851135 [Halomonas sp. FeN2]
ATTATCGAGTTGATTAAAACTGATGTCTTCGATAGATGGCTACGCGAGCTTCGCGATGTTCGTGCCCGCGCCAAGGTTGA
AGCCCGCATCCGACGTTTGAGTCTGGGCAACCCTGGAGATGTGAAGCCGGTAGGTAAAGGCGTTTCTGAACTGCGAATTC
CTTATGGGCCAGGCTGCCGCATCTATTATATTACCAAGGGGCCAGTAATCGTGGTTCTGCTGTGCGGTGGTGATAAAGCC
ACCCAATCCCACGATATCGAACTAGCCAAAGCCATTGCCGCGCAATGGAAGGAGTAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2