Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 49702..50500 Replicon plasmid pAMPD01
Accession NZ_CP058309
Organism Escherichia coli strain AMSCJX04

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag HXS78_RS23490 Protein ID WP_000072677.1
Coordinates 49702..50223 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag HXS78_RS23495 Protein ID WP_001351987.1
Coordinates 50231..50500 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS78_RS23470 46306..48048 + 1743 Protein_53 phage tail protein -
HXS78_RS23475 48251..48574 + 324 WP_001717323.1 hypothetical protein -
HXS78_RS23480 48588..49280 + 693 WP_000856757.1 hypothetical protein -
HXS78_RS23485 49282..49533 + 252 WP_000901559.1 hypothetical protein -
HXS78_RS23490 49702..50223 - 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
HXS78_RS23495 50231..50500 - 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
HXS78_RS23500 50819..51487 + 669 WP_000161228.1 AAA family ATPase -
HXS78_RS23505 51493..51846 + 354 WP_160378290.1 hypothetical protein -
HXS78_RS23510 51899..52666 - 768 WP_000342417.1 hypothetical protein -
HXS78_RS23515 52949..53689 + 741 WP_177754127.1 hypothetical protein -
HXS78_RS23520 53733..55073 + 1341 WP_000137333.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..109907 109907


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T166511 WP_000072677.1 NZ_CP058309:c50223-49702 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T166511 NZ_CP074185:c6177-5872 [Raoultella planticola]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT166511 WP_001351987.1 NZ_CP058309:c50500-50231 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT166511 NZ_CP074185:c6397-6179 [Raoultella planticola]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References