Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4340472..4340969 Replicon chromosome
Accession NZ_CP058308
Organism Escherichia coli strain AMSCJX04

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HXS78_RS20785 Protein ID WP_001173343.1
Coordinates 4340472..4340645 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HXS78_RS20790 Protein ID WP_000398619.1
Coordinates 4340673..4340969 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS78_RS20775 4337142..4338599 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HXS78_RS20780 4338836..4340353 + 1518 WP_001295074.1 lysine--tRNA ligase -
HXS78_RS20785 4340472..4340645 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HXS78_RS20790 4340673..4340969 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HXS78_RS20795 4341196..4341468 - 273 WP_000405647.1 N-acetyltransferase -
HXS78_RS20800 4341480..4341710 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
HXS78_RS20805 4341891..4343522 + 1632 WP_001216461.1 sensor histidine kinase -
HXS78_RS20810 4343519..4344238 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166509 WP_001173343.1 NZ_CP058308:c4340645-4340472 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166509 NZ_CP074182:c5172786-5172472 [Raoultella planticola]
ATGCAGTTCACCGTATACGGTAATACCGGTAAAAGCGCCGTTTACCCCCTGTTGCTTGATGTCACAAGCGATATCATTGG
ACAACTGAACCGCCGGATCGTGATCCCGTTGTTGCCTGTTGACAGGTATCCGGCAGGCCGCCGCCCGGATCGTCTTGTCC
CCGTGGTCAGACTGACTGACGGCCAGGAATACGCGGTCATGACCCACGAACTGGCGAGTGTCCCTGTCCGGGCGCTGGGG
GCGGTGTTCTGCGATGCGTCGCAGTACCGCACTCAGGTGAAGGCCGCCATCGATTTCCTCATTGACGGCATTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT166509 WP_000398619.1 NZ_CP058308:c4340969-4340673 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT166509 NZ_CP074182:c5173025-5172789 [Raoultella planticola]
ATGACCGCGAAACAACGCAACACACTGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAGGCGGG
TATTAACCTGAGTGCGACCCTGACCGCCGCCCTGGATGCGGAGCTTCGTCACCATGAAGCCAAAAAATGGCAGGAAGAGA
ACAGGGAGGCCATCGACGCATTAAACCGTTTCCATGATGAGTATGGCTGTTTCAGTGATGAATACCGGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References