Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3527692..3528529 Replicon chromosome
Accession NZ_CP058308
Organism Escherichia coli strain AMSCJX04

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HXS78_RS16940 Protein ID WP_000227784.1
Coordinates 3527987..3528529 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HXS78_RS16935 Protein ID WP_001297137.1
Coordinates 3527692..3528003 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS78_RS16910 3522712..3523659 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HXS78_RS16915 3523681..3525672 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HXS78_RS16920 3525662..3526276 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HXS78_RS16925 3526276..3526605 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HXS78_RS16930 3526617..3527507 + 891 WP_000971336.1 heme o synthase -
HXS78_RS16935 3527692..3528003 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HXS78_RS16940 3527987..3528529 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HXS78_RS16945 3528585..3529520 - 936 WP_001297127.1 sel1 repeat family protein -
HXS78_RS16950 3529928..3531292 + 1365 WP_001000978.1 MFS transporter -
HXS78_RS16955 3531420..3531911 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HXS78_RS16960 3532079..3532990 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T166506 WP_000227784.1 NZ_CP058308:3527987-3528529 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T166506 NZ_CP074182:2058304-2058407 [Raoultella planticola]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT166506 WP_001297137.1 NZ_CP058308:3527692-3528003 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT166506 NZ_CP074182:c2058412-2058246 [Raoultella planticola]
CAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGCGTCAGGTTTTCCAGTCCGCAACAAAAGTGGT
CGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References