Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2521808..2522446 Replicon chromosome
Accession NZ_CP058308
Organism Escherichia coli strain AMSCJX04

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HXS78_RS12110 Protein ID WP_000813794.1
Coordinates 2522270..2522446 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HXS78_RS12105 Protein ID WP_001270286.1
Coordinates 2521808..2522224 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS78_RS12085 2516960..2517901 - 942 WP_001251319.1 ABC transporter permease -
HXS78_RS12090 2517902..2518915 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HXS78_RS12095 2518933..2520078 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
HXS78_RS12100 2520323..2521729 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
HXS78_RS12105 2521808..2522224 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HXS78_RS12110 2522270..2522446 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HXS78_RS12115 2522668..2522898 + 231 WP_000494244.1 YncJ family protein -
HXS78_RS12120 2522990..2524951 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HXS78_RS12125 2525024..2525560 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
HXS78_RS12130 2525613..2526827 + 1215 WP_001349372.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2526867..2528132 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T166503 WP_000813794.1 NZ_CP058308:c2522446-2522270 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T166503 NZ_CP074179:194959-195369 [Enterobacter asburiae]
GTGAACAAGACCTATATGCTCGACACGAACATCTGCTCATTCATCATGCGCGAACAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTGGTCTGAGATTTCATTCGCCGCCCGGGCTG
CAGGGCCGGTCACGCAGCAGCTGGCTGAAGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCCGCGGTG
GATGCGACCACGGAGGTAAAGGTGGCGCTGCGCCTCGCCGGGACGCCGCCAGGCCCGAACGACACGGCGATAGCCGGGCA
CGCCATCGCCGCCGGCGCGGTGCTGGTGACGAGGATCGGGGGAGAGTTTGAGCGGGTGCCGGGGTTAATGCTTGAAGACT
GGGTGAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT166503 WP_001270286.1 NZ_CP058308:c2522224-2521808 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT166503 NZ_CP074179:194732-194962 [Enterobacter asburiae]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGCGTCAG
CGAGCTGGTGATTGTCCGGGAAGGAGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References