Detailed information of TA system TA166491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1366741..1367366
• Replicon: chromosome
• Accession: NZ_CP058308
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HXS78_RS06605
• Protein ID: WP_000911330.1
• Coordinates: 1366968..1367366 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HXS78_RS06600
• Protein ID: WP_000450524.1
• Coordinates: 1366741..1366968 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS06575	1362544..1363014	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HXS78_RS06580	1363014..1363586	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HXS78_RS06585	1363732..1364610	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HXS78_RS06590	1364627..1365661	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HXS78_RS06595	1365874..1366587	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HXS78_RS06600	1366741..1366968	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HXS78_RS06605	1366968..1367366	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HXS78_RS06610	1367513..1368376	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
HXS78_RS06615	1368391..1370406	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
HXS78_RS06620	1370480..1371178	+	699	WP_000679823.1	esterase	-
HXS78_RS06625	1371288..1371488	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T166491 WP_000911330.1 NZ_CP058308:1366968-1367366 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T166491 NZ_CP074177:1083562-1083912 [Enterobacter asburiae]
ATGTGGACGGTTCTGTTCGGAAAGGTATTCGAGCAATGGCTGCTTGAGCAGGAAGAAGGATTACAGGATAAGGTACTGGC
AGACCTGTTAAATCTACAAAACTATGGGCCACGTCTTCCTCGTCCCTATGCAGATACGGTTAAAGGCTCACGGTATAACC
ATATGAAGGAATTACGTATTCAATATGCTGGTCGTCCGATACGTGCTTTCTTCGCATTTGATCCTGTCCGTCAGGCCATT
GTGCTTTGCGCGGGCGATAAGAGTAACGACAAAGCCTTTTATGAAAAAATGATTCGCATCGCAGATGCCGAGTTTTTCTT
TCATCTGACAAGCCTGGAGGCAACTAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT166491 WP_000450524.1 NZ_CP058308:1366741-1366968 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT166491 NZ_CP074177:1083909-1084208 [Enterobacter asburiae]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGTTCACCGGAAAGCCAACAACGTATTAAAGAAATGGCAGATGAAATGAT
CCTCGAGACGGGTCTGCAACTTTTACGTGAAGAGCTGAGGCTTTCGCAAAAAAATGTTGCAGATCTGATGGGGGTGAGCC
AGCCCGCAGTAACGCAGCTAGAGCAACGTGGAAATGAACTCAAAATTGCCACGCTTAAGCGCTACATTGAGGCAATAGGG
GGGAAACTTCATATCGACATTGAACTTCCCACAGGAAAACGCGTTGCATTTCATATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33