Detailed information of TA system TA166489

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 932429..933083
• Replicon: chromosome
• Accession: NZ_CP058308
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: F4NJ21
• Locus tag: HXS78_RS04540
• Protein ID: WP_000244772.1
• Coordinates: 932676..933083 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HXS78_RS04535
• Protein ID: WP_063100142.1
• Coordinates: 932429..932695 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS04510	927717..928460	+	744	WP_000951964.1	SDR family oxidoreductase	-
HXS78_RS04515	928517..929950	-	1434	WP_001363803.1	6-phospho-beta-glucosidase BglA	-
HXS78_RS04520	929995..930306	+	312	WP_001182944.1	N(4)-acetylcytidine aminohydrolase	-
HXS78_RS04525	930470..931129	+	660	WP_000250274.1	hemolysin III family protein	-
HXS78_RS04530	931206..932186	-	981	WP_000886062.1	tRNA-modifying protein YgfZ	-
HXS78_RS04535	932429..932695	+	267	WP_063100142.1	FAD assembly factor SdhE	Antitoxin
HXS78_RS04540	932676..933083	+	408	WP_000244772.1	protein YgfX	Toxin
HXS78_RS04545	933123..933644	-	522	WP_001055874.1	flavodoxin FldB	-
HXS78_RS04550	933756..934652	+	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
HXS78_RS04555	934677..935387	+	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HXS78_RS04560	935393..937126	+	1734	WP_000813215.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16048.02 Da
• Isoelectric Point: 11.2511

>T166489 WP_000244772.1 NZ_CP058308:932676-933083 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T166489 NZ_CP074177:398918-399295 [Enterobacter asburiae]
ATGCAAACCTTATCCGCCATTCCGGCTCGGAAGGCACCATCACACCCAACGCCCGTTGAAATCTGGCAGCGACTTCTTAC
GTATCTGCTGGAAAGGCATTACGGCCTTTCGCTTAACGACACACAGTTTGGCAACGACGGTGTCATTCAGCAGCATATTG
ACGCCGGGCTTTCACTGGCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTACGAATTGATCGCCCCGGTTTC
TCCATTCAGCATCAGTCTCCGTTCATCAGCGCTATTGATATACTCCGGGCCAGAAAGGCCATCGGCCTGATGCAACGTAC
CGGTTATAAAGCGGTGACTTGCGCCATCAGTGGACGCTCTTCCGGAGGGCTGAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10548.07 Da
• Isoelectric Point: 5.1599

>AT166489 WP_063100142.1 NZ_CP058308:932429-932695 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFNRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT166489 NZ_CP074177:398496-398861 [Enterobacter asburiae]
ATGCCGAACACAACACCTGTAGCAAACCATGATATTTCTGAGCCCTGGTGGGGACTTAAACCCACCGTCACGCCATGCTT
TGGCGCACGACTGGTACAGGAAGGCAACCACCTGCATTATCTGGCTGACCGCAGCACTATCGAAGGAACATTCAGCGACG
CGGATTTACGTCATCTCGACCAGGCCTTTCCGGTACTAATGAAGCAGCTTGAGTTAATGCTGGTTTCCGGTGAACTCAAT
CCCCGCAATCAGCATTGCGTCACGTTGTATGCAAAAGGTCTGACCCTCGACGCAGATACACTGGGCAGTTGTGGTTACCT
TTATATCGCGATTTATCCGACGTCAGGCAACAGCATCCCCCGCTAA

Structures

Toxin

• AlphaFold DB: A0A0E0XYB4