Detailed information of TA system TA166483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 902616..904249
• Replicon: chromosome
• Accession: NZ_CP058307
• Organism: Avibacterium paragallinarum strain AVPG2015

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A3G8W245
• Locus tag: VY92_RS04345
• Protein ID: WP_035684858.1
• Coordinates: 902942..904249 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: VY92_RS04340
• Protein ID: WP_081637498.1
• Coordinates: 902616..902942 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VY92_RS04320	898118..898849	+	732	WP_035684848.1	class I SAM-dependent methyltransferase	-
VY92_RS04325	898956..900725	+	1770	WP_035684851.1	30S ribosomal protein S12 methylthiotransferase accessory factor YcaO	-
VY92_RS04330	900860..901282	+	423	WP_035684853.1	DUF1043 family protein	-
VY92_RS04335	901445..902575	+	1131	WP_035684855.1	ribonucleotide-diphosphate reductase subunit beta	-
VY92_RS04340	902616..902942	-	327	WP_081637498.1	helix-turn-helix domain-containing protein	Antitoxin
VY92_RS04345	902942..904249	-	1308	WP_035684858.1	HipA domain-containing protein	Toxin
VY92_RS04350	904349..904597	-	249	WP_035684861.1	2Fe-2S ferredoxin-like protein	-
VY92_RS04355	905872..906684	-	813	WP_035684866.1	4-hydroxy-tetrahydrodipicolinate reductase	-
VY92_RS04360	906699..907334	-	636	WP_035684869.1	LysE family transporter	-
VY92_RS04365	907346..907846	-	501	WP_017806513.1	phosphatidylglycerophosphatase A	-
VY92_RS04370	907852..908886	-	1035	WP_035684872.1	thiamine-phosphate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-351)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50363.28 Da
• Isoelectric Point: 5.6627

>T166483 WP_035684858.1 NZ_CP058307:c904249-902942 [Avibacterium paragallinarum]
MEKLTLQTYIDQEWRDMAELHFEKETYQLSNLVYFPDYITQYFGQEGSRAVSINYPVHIFIENRKTLWSCIGDIAPAGAS
RRYWIESLDIKHLPESEQNYRLLKYGAISPVGNLRIKEATPQKTEVRYFDIEKVKDRQSDFLEYANQSGAMVGGATGAGG
EAPKLLLKRHNGKVWIDNLQDGKDQDISYLVKYPRGRYSPIDCDILRAEFHYYQEVAELGFDTIDIHHMCLEEGERYPSL
WLPRFDITKKEGKYNRLALESVYSMLQKQGGTLEHEVTIRQLIKIISASNLEFDQQHFVLEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQKIWLAPIYDFAPMRADPEGIIRSTTWNRKGSLPQEFAGEYRFDLIAESLSDLIEPSLLLTELQQLAIRLVD
LKSRLAQRGVPQAILDHPSIGFDYLPEKLKAWGLV

Nucleotide

• Length: 1308 bp

>T166483 NZ_CP074172:c85185-84898 [Enterobacter sp. JBIWA003]
ATGACTTATACAGTCAAATTCAGGGACGATGCATTGAAAGAATGGCAAAAGCTGGACAAGCCCATTCAGCAGCAGTTTGC
AAAAAAGCTGAAGAAGTGCAGTGAAAATCCGCATATCCCTTCAGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATCA
AACTTCGAGCGTCTGGCTTTCGTCTGGTCTATCAGGTGATTGACGATCAATTAATTATCGCTGTTGTAGCTGTAGGAAAA
CGTGAACGCAGTGAAGTTTATAATCTTGCCAGTGAACGAATGAGGTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12252.39 Da
• Isoelectric Point: 10.9810

>AT166483 WP_081637498.1 NZ_CP058307:c902942-902616 [Avibacterium paragallinarum]
MITRKNASKVGATISTTERNNVRNRIIKALLLGKMTQGQALRKLRIEMLGVNQQQYMKVVKVSRQTLSNIENDKGNYSVE
TINQVFKPFGFELGLVPISKELLTEIMQ

Nucleotide

• Length: 327 bp

>AT166483 NZ_CP074172:c85433-85182 [Enterobacter sp. JBIWA003]
ATGCCCAACATCATTCTCAGTGACACAAGTGCCAGTGTCAGCGAACTCAAGAAAAACCCTATGGCAACGGTCAGCGCTGG
TGATGGCTACCCGGTTGCTATTCTGAACCGCAACCAGCCAGCTTTTTACTGTGTTCCGGCAGAGCTGTACGAGAAGATGC
TCGATGCCCTCGACGATCAGGAACTGGTTAAACTTGTCGCAGAACGCAGCAGCCAGACGCTTCACGACGTGGATCTGGAT
AGCTACCTATGA

Structures

Toxin

• AlphaFold DB: A0A3G8W245