Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17526..18169 Replicon plasmid pAH65-3
Accession NZ_CP058305
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HUU85_RS23710 Protein ID WP_001044768.1
Coordinates 17526..17942 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HUU85_RS23715 Protein ID WP_001261287.1
Coordinates 17939..18169 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS23695 14025..14615 - 591 WP_000194575.1 hypothetical protein -
HUU85_RS23700 14615..14872 - 258 WP_000343085.1 hypothetical protein -
HUU85_RS23705 15226..17364 + 2139 WP_000350638.1 AAA family ATPase -
HUU85_RS23710 17526..17942 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HUU85_RS23715 17939..18169 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HUU85_RS23720 18465..18755 + 291 WP_000111771.1 hypothetical protein -
HUU85_RS23725 18745..19644 + 900 WP_000963206.1 nucleotide-binding protein -
HUU85_RS23730 19694..21919 - 2226 WP_000698737.1 phage T7 exclusion protein -
HUU85_RS23735 21921..23009 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / sitABCD / floR / aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / mph(A) iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..139513 139513


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T166471 WP_001044768.1 NZ_CP058305:c17942-17526 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T166471 NZ_CP074170:645003-645344 [Enterobacter sp. JBIWA003]
ATGAAAACCTTACCTGTAACAACTCAGCGGGCGACTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGCCTGCTGGAACAACATTATGGCCTTACCCTGAATGACACGCTGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCCGGTATCACACTGGCTGATGCCGTTAACTTTCTAGTGGAAAAGTACGAACTGGTGCGTATTGATCGCAGGGGGGTT
AATTGTCAGGAGCAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGGCAGGCAACCGGTCTGTTGCGATATCG
TCATAACACCCCGTCGCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT166471 WP_001261287.1 NZ_CP058305:c18169-17939 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT166471 NZ_CP074170:644661-644978 [Enterobacter sp. JBIWA003]
ATCGCTGACCCCTGGTGGGGACTTAAGCCTGCAGCCATCCCGTGTTTTGGCGCTCGTCTGGTGCAGGAGGGCAACCGACT
GCACTACATGGCTGACCGGGCCAGTATCACCGGTATATTTGGTGATGCTGAATGTCTGAAGCTGGAAGAAGCATTTCCGC
ATTTTATCCGCCAGATTGAATTGTCGCTGAACACTGGTGAACTAAAAGCTCGTCATGCCCACTGCGTTACTTTGTACCAC
AATGCTTTCACCTGTGAAGCCGATACTCTCGGCAGTTGTGGGTACGTCTACATCGTTATTTATCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References