Detailed information of TA system TA166466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1981..2540
• Replicon: plasmid pAH65-2
• Accession: NZ_CP058304
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: HUU85_RS23165
• Protein ID: WP_000421259.1
• Coordinates: 2265..2540 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: HUU85_RS23160
• Protein ID: WP_001178087.1
• Coordinates: 1981..2265 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS23155	46..1077	+	1032	WP_000907852.1	replication initiation protein	-
HUU85_RS23160	1981..2265	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
HUU85_RS23165	2265..2540	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUU85_RS23170	2646..2927	+	282	WP_001105066.1	hypothetical protein	-
HUU85_RS23175	3119..3385	-	267	WP_000627832.1	hypothetical protein	-
HUU85_RS23180	3385..3879	-	495	WP_015058884.1	hypothetical protein	-
HUU85_RS23185	3935..4537	-	603	WP_000521606.1	hypothetical protein	-
HUU85_RS23190	4713..5765	-	1053	WP_000655519.1	hypothetical protein	-
HUU85_RS23195	5978..6568	-	591	WP_001401589.1	hypothetical protein	-
HUU85_RS23200	6568..6822	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84033	84033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T166466 WP_000421259.1 NZ_CP058304:2265-2540 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T166466 NZ_CP074168:c4735429-4735154 [Serratia ureilytica]
ATGCTCAACATCGAATGGACTCGAGCCGCACGTCAGGATCTGGCGGAGATTGTGGGATTCATCGCCAACGATAACCCTCT
GGCGGCCAGAAAAATGAAGGTTCTCATCATGGAGGCCGTCACTCCCGCCGCCAGGCATCCTCGAATTTTCCGGCGTGGCA
GAGTGGACGGCACGCATGAAATCCCCTTGCATCCCCATTATCTCCTGATTTACCAGGTGCTGGAAACTCACATCCGGGTG
TTGCGGGTATTACATACGCGCCGACAATATCCTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT166466 WP_001178087.1 NZ_CP058304:1981-2265 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT166466 NZ_CP074168:c4735629-4735429 [Serratia ureilytica]
ATGATGAGTATTTTACTACTGCCTACGGTTGCCAAGATGGAAAATGAGGAACACACACAGGGCTATGAGCGCTGGTTTCT
TGAAAGAGTTCAGGAAGCCATGGATGAGCCGTGGCCCGGTATTCCTCATGATGACGTGATGGCTGAAATGGATGCTGTTA
TCGCTGCTGCAGAAGCCAGGATCAACGCGAAGCAGGCATAA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625