Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 7053..7546 Replicon plasmid pAH65-1
Accession NZ_CP058303
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag HUU85_RS22845 Protein ID WP_001230709.1
Coordinates 7053..7307 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag HUU85_RS22850 Protein ID WP_000151456.1
Coordinates 7304..7546 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS22810 2628..2789 + 162 WP_001366886.1 type I toxin-antitoxin system Hok family toxin -
HUU85_RS22815 2939..3535 + 597 WP_000125165.1 hypothetical protein -
HUU85_RS22820 3553..3723 - 171 WP_000186151.1 hypothetical protein -
HUU85_RS22825 3911..4576 - 666 WP_000532149.1 P-loop NTPase -
HUU85_RS22830 5165..5494 + 330 WP_001542022.1 hypothetical protein -
HUU85_RS22835 5895..6410 + 516 WP_001025393.1 J domain-containing protein -
HUU85_RS22840 6514..7056 + 543 WP_001083904.1 hypothetical protein -
HUU85_RS22845 7053..7307 + 255 WP_001230709.1 hypothetical protein Toxin
HUU85_RS22850 7304..7546 + 243 WP_000151456.1 hypothetical protein Antitoxin
HUU85_RS22855 8117..8410 + 294 WP_000673428.1 hypothetical protein -
HUU85_RS22860 8899..9147 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
HUU85_RS22865 9144..9491 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
HUU85_RS22870 9579..9761 - 183 WP_001428470.1 hypothetical protein -
HUU85_RS22875 10263..10562 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
HUU85_RS22880 10822..11148 - 327 WP_000206697.1 hypothetical protein -
HUU85_RS22885 11150..11590 - 441 WP_023223352.1 hypothetical protein -
HUU85_RS22890 11610..12317 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..60960 60960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-99)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T166464 WP_001230709.1 NZ_CP058303:7053-7307 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T166464 NZ_CP074168:c3356398-3356096 [Serratia ureilytica]
ATTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGATGTCCT
CTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAACGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGGAGATTG
AGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCCGTTGGT
AATGTCAATGAGCACAGGAATCAAATTAAAGCCGCTATCGATTTTCTGATTGATGGCTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT166464 WP_000151456.1 NZ_CP058303:7304-7546 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT166464 NZ_CP074168:c3356644-3356408 [Serratia ureilytica]
ATGGCAAATAGCAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCCGTATTGACAGACGCATTGAGCGTCCGCGTCAGGGATATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGCGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References