Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4727004..4728592 Replicon chromosome
Accession NZ_CP058302
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag HUU85_RS22595 Protein ID WP_176256532.1
Coordinates 4727004..4728326 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag HUU85_RS22600 Protein ID WP_001301023.1
Coordinates 4728326..4728592 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS22575 4722936..4723316 + 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
HUU85_RS22580 4723313..4723660 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
HUU85_RS22585 4723710..4725248 + 1539 WP_176256509.1 IS66 family transposase -
HUU85_RS22590 4725354..4726229 - 876 WP_001059072.1 site-specific integrase -
HUU85_RS22595 4727004..4728326 - 1323 WP_176256532.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
HUU85_RS22600 4728326..4728592 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
HUU85_RS22605 4728815..4730215 - 1401 WP_032083678.1 autotransporter outer membrane beta-barrel domain-containing protein -
HUU85_RS22610 4730212..4733520 - 3309 Protein_4404 autotransporter barrel domain-containing lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4691019..4735011 43992


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49305.84 Da        Isoelectric Point: 8.2482

>T166463 WP_176256532.1 NZ_CP058302:c4728326-4727004 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHTKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T166463 NZ_CP074168:c2953611-2953515 [Serratia ureilytica]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT166463 WP_001301023.1 NZ_CP058302:c4728592-4728326 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT166463 NZ_CP074168:2953515-2953655 [Serratia ureilytica]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References