Detailed information of TA system TA166458

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4229157..4230001
• Replicon: chromosome
• Accession: NZ_CP058302
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: HUU85_RS20085
• Protein ID: WP_000854686.1
• Coordinates: 4229618..4230001 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: HUU85_RS20080
• Protein ID: WP_001285602.1
• Coordinates: 4229157..4229537 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS20045	4225407..4225861	+	455	Protein_3897	hypothetical protein	-
HUU85_RS20050	4225940..4226173	+	234	WP_001119719.1	DUF905 family protein	-
HUU85_RS20055	4226273..4227091	+	819	WP_001234732.1	DUF945 domain-containing protein	-
HUU85_RS20060	4227183..4227668	+	486	WP_000214307.1	antirestriction protein	-
HUU85_RS20065	4227684..4228160	+	477	WP_001186726.1	RadC family protein	-
HUU85_RS20070	4228223..4228444	+	222	WP_000692298.1	DUF987 domain-containing protein	-
HUU85_RS20075	4228463..4229146	+	684	WP_000086769.1	hypothetical protein	-
HUU85_RS20080	4229157..4229537	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUU85_RS20085	4229618..4230001	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
HUU85_RS20090	4229998..4230486	+	489	WP_001054233.1	hypothetical protein	-
HUU85_RS20095	4230503..4230700	+	198	WP_000839253.1	DUF957 domain-containing protein	-
HUU85_RS20100	4230785..4231627	+	843	WP_001274547.1	DUF4942 domain-containing protein	-
HUU85_RS20110	4232098..4233036	+	939	WP_000351315.1	glyoxylate/hydroxypyruvate reductase GhrA	-
HUU85_RS20115	4233091..4233828	+	738	WP_000283664.1	zinc-binding phosphatase	-
HUU85_RS20120	4233852..4234406	+	555	WP_001001902.1	molecular chaperone YcdY	-
HUU85_RS20125	4234508..4234999	+	492	WP_001300785.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	4194565..4240565	46000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T166458 WP_000854686.1 NZ_CP058302:4229618-4230001 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T166458 NZ_CP074168:c480549-480259 [Serratia ureilytica]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGAAGGCAGGCGCAGCCGTCTTATCCCCGGCGCGCT
CGAGCGGCAGTTGGCCAGAAAGCTCGATATGTTAGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAGCGGCTATCCGGGCCATTTCAGGGATGGTCGAGTCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCCGAAAAAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT166458 WP_001285602.1 NZ_CP058302:4229157-4229537 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT166458 NZ_CP074168:c480244-479921 [Serratia ureilytica]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGAGAAGTCCTGCAGGAAGAATATTTAACGCCGTTAGGCATCGG
CCAAGAAGAACTGGCAAAGGTATTGGGCGTCACCCGCGCCACCGTCAATCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAATCTGTTTGCCGATCTGTTCGAAACGACCGCCGACTTTTGGCTAAACCTGCAGGCGGCACACGATCGC
TGGGAAGCCAGACAGGCCGATGCGCAGCGTGTGCGGCTGCGGCCAATCAAAGAAATACTGGGACGCAATATCACTCATGC
ATGA