Detailed information of TA system TA166457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4208437..4210157
• Replicon: chromosome
• Accession: NZ_CP058302
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: HUU85_RS19990
• Protein ID: WP_000312833.1
• Coordinates: 4209501..4210157 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: HUU85_RS19985
• Protein ID: WP_000634203.1
• Coordinates: 4208437..4209504 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS19970	4203658..4204251	-	594	Protein_3882	IS3-like element IS600 family transposase	-
HUU85_RS19975	4204607..4205323	-	717	WP_001122107.1	M48 family metallopeptidase	-
HUU85_RS19980	4205323..4208418	-	3096	WP_000108735.1	HsdR family type I site-specific deoxyribonuclease	-
HUU85_RS19985	4208437..4209504	-	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
HUU85_RS19990	4209501..4210157	-	657	WP_000312833.1	DUF4433 domain-containing protein	Toxin
HUU85_RS19995	4210170..4211504	-	1335	WP_000228013.1	restriction endonuclease subunit S	-
HUU85_RS20000	4211504..4213942	-	2439	WP_000985465.1	type I restriction-modification system subunit M	-
HUU85_RS20005	4213982..4214221	-	240	WP_001431807.1	hypothetical protein	-
HUU85_RS20010	4214503..4214757	+	255	WP_154670580.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	4194565..4240565	46000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T166457 WP_000312833.1 NZ_CP058302:c4210157-4209501 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T166457 NZ_CP074168:c417289-416978 [Serratia ureilytica]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGGGGTGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAGTACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTAGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCGTCATGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT166457 WP_000634203.1 NZ_CP058302:c4209504-4208437 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT166457 NZ_CP074168:c416981-416559 [Serratia ureilytica]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTACCCTTATTGCAGGGCAGCCACGCTCAGCAAGA
CTACCGAGAAGCGCTCGAATTGGTGGAATATCTGCTGGAAAACGATGAACGCCATCCGCTGATTGACATGTTGGCAAAAA
AAATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAACTCCCGCAGGGGGTGGCC
GCGCTTCGCGTATTGATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGCAAAAAGTCGTTGGTCAA
CCAAATCCTGAACGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGCTTCAATCTGCCCCCAGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9