Detailed information of TA system TA166453

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2942239..2942946
• Replicon: chromosome
• Accession: NZ_CP058302
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A377AQ31
• Locus tag: HUU85_RS13980
• Protein ID: WP_032161355.1
• Coordinates: 2942605..2942946 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUU85_RS13975
• Protein ID: WP_032161354.1
• Coordinates: 2942239..2942574 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS13950	2938186..2938404	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
HUU85_RS13955	2938522..2939136	-	615	WP_087901029.1	inovirus Gp2 family protein	-
HUU85_RS13960	2939729..2940682	+	954	WP_032161351.1	hypothetical protein	-
HUU85_RS13965	2940918..2941736	+	819	WP_001683514.1	DUF945 domain-containing protein	-
HUU85_RS13970	2941766..2942239	+	474	WP_032161353.1	DNA repair protein RadC	-
HUU85_RS13975	2942239..2942574	+	336	WP_032161354.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUU85_RS13980	2942605..2942946	+	342	WP_032161355.1	TA system toxin CbtA family protein	Toxin
HUU85_RS13985	2943061..2943894	+	834	WP_032250394.1	DUF4942 domain-containing protein	-
HUU85_RS13990	2943971..2944219	+	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
HUU85_RS13995	2944223..2944498	+	276	WP_001683517.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HUU85_RS14000	2944615..2945403	+	789	WP_032161357.1	helix-turn-helix domain-containing protein	-
HUU85_RS14005	2946317..2946967	+	651	WP_001037977.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE	2927947..2952994	25047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13048.01 Da
• Isoelectric Point: 9.8953

>T166453 WP_032161355.1 NZ_CP058302:2942605-2942946 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLTRLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRSHNTTAR

Nucleotide

• Length: 342 bp

>T166453 NZ_CP074161:c58405-57995 [Enterobacter sp. JBIWA005]
GTGAACAAGACCTATATGCTCGACACGAACATCTGCTCATTCATCATGCGCGAACAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTGGTCTGAGATTTCATTCGCCGCCCGGGCTG
CAGGGCCGGTCACGCAGCAGCTGGCTGAAGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCCGCGGTG
GATGCGACCACGGAGGTAAAGGTGGCGCTGCGCCTCGCCGGGACGCCGCCAGGCCCGAACGACACGGCGATAGCCGGGCA
CGCCATCGCCGCCGGCGCGGTGCTGGTGACGAGGATCGGGGGAGAGTTTGAACGGGTACCGGGGTTAATGCTTGAAGACT
GGGTGAAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12530.56 Da
• Isoelectric Point: 8.1281

>AT166453 WP_032161354.1 NZ_CP058302:2942239-2942574 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSACQKR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT166453 NZ_CP074161:c58632-58402 [Enterobacter sp. JBIWA005]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGCGTCAG
CGAGCTGGTGATTGTCCGGGAAGGAGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A377AQ31