Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2734452..2735321 Replicon chromosome
Accession NZ_CP058302
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M1ISH3
Locus tag HUU85_RS12970 Protein ID WP_039023580.1
Coordinates 2734452..2734862 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0X5FMN2
Locus tag HUU85_RS12975 Protein ID WP_039023579.1
Coordinates 2734953..2735321 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS12955 2731047..2732585 - 1539 WP_021578236.1 lysine decarboxylation/transport transcriptional activator CadC -
HUU85_RS12960 2733422..2733982 - 561 Protein_2514 DUF4942 domain-containing protein -
HUU85_RS12965 2734127..2734489 - 363 WP_072650913.1 DUF957 domain-containing protein -
HUU85_RS12970 2734452..2734862 - 411 WP_039023580.1 TA system toxin CbtA family protein Toxin
HUU85_RS12975 2734953..2735321 - 369 WP_039023579.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HUU85_RS12980 2735371..2735645 - 275 Protein_2518 antitoxin of the YeeV-YeeU toxin-antitoxin system -
HUU85_RS12985 2735743..2736003 - 261 WP_032303908.1 hypothetical protein -
HUU85_RS12990 2736018..2736239 - 222 WP_000692315.1 DUF987 domain-containing protein -
HUU85_RS12995 2736302..2736778 - 477 WP_176256622.1 RadC family protein -
HUU85_RS13000 2736794..2737273 - 480 WP_001696591.1 antirestriction protein -
HUU85_RS13005 2737539..2738357 - 819 WP_001175153.1 DUF945 domain-containing protein -
HUU85_RS13010 2738447..2738680 - 234 WP_001278287.1 DUF905 family protein -
HUU85_RS13015 2738686..2739363 - 678 WP_001097368.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2723234..2776000 52766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15253.38 Da        Isoelectric Point: 7.2150

>T166452 WP_039023580.1 NZ_CP058302:c2734862-2734452 [Escherichia coli]
MKLLPDTHVREASCCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITLGSIRGRNDETFPDAGSRQR

Download         Length: 411 bp

>T166452 NZ_CP074161:37589-37855 [Enterobacter sp. JBIWA005]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGATAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13672.49 Da        Isoelectric Point: 6.7393

>AT166452 WP_039023579.1 NZ_CP058302:c2735321-2734953 [Escherichia coli]
VSDTLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT166452 NZ_CP074161:37381-37605 [Enterobacter sp. JBIWA005]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M1ISH3

Antitoxin

Source ID Structure
AlphaFold DB A0A0X5FMN2

References