Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2723234..2723731 Replicon chromosome
Accession NZ_CP058302
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUU85_RS12930 Protein ID WP_001173343.1
Coordinates 2723558..2723731 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUU85_RS12925 Protein ID WP_000398619.1
Coordinates 2723234..2723530 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS12905 2719965..2720684 - 720 WP_000611288.1 two-component system response regulator DcuR -
HUU85_RS12910 2720681..2722312 - 1632 WP_001565376.1 sensor histidine kinase -
HUU85_RS12915 2722493..2722723 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
HUU85_RS12920 2722735..2723007 + 273 WP_000405647.1 N-acetyltransferase -
HUU85_RS12925 2723234..2723530 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUU85_RS12930 2723558..2723731 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUU85_RS12935 2723850..2725367 - 1518 WP_001295074.1 lysine--tRNA ligase -
HUU85_RS12940 2725604..2727061 - 1458 WP_176256623.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2723234..2776000 52766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166451 WP_001173343.1 NZ_CP058302:2723558-2723731 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166451 NZ_CP074160:165378-165653 [Enterobacter sp. JBIWA005]
TTGTCGAAGTGGAATATTAAGTTCACCAGGAACGGAGCTGATGACCTTAGCTACTGGCGTGATACTGACAAAAAGAAGTT
TGAGCGTATCCGGCAGCTGTTGCGCAGCATCGAGGCTGATCCGCTGACAGGTATCGGAAAACCCGAACGGCTGCGGCACC
ACAAAGATCCGGCGCTCTATTCCCGCCGGATTGATCAGAGTCACCGTCTGGTCTATTCAATCAAGAGCGGAGAAATCATC
ATCTACGCAGCCCGTTATCACTATGGTGACAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT166451 WP_000398619.1 NZ_CP058302:2723234-2723530 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT166451 NZ_CP074160:165152-165397 [Enterobacter sp. JBIWA005]
ATGCATGCAATCAATTTTACTGATGCCCGTAAACATTTTGCTGACACCATGAAGCGCGTCACTGATGATGCTGAACCTGT
ACGCGTCATGCGTCGTGACGCACCTGATATTGTTATGATCGATGCAGGAGAGTATGAGGCGCTAATCGAAACGGTCTATC
TGTTCAGCAATCCGGCGAACGCCGCGCATATCAATGAGTCAATGAAACAGGCGGAGCGCGGCGAATTTGTCGAAGTGGAA
TATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References