Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1396283..1396937 Replicon chromosome
Accession NZ_CP058302
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HUU85_RS06670 Protein ID WP_000244777.1
Coordinates 1396283..1396690 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUU85_RS06675 Protein ID WP_000354046.1
Coordinates 1396671..1396937 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS06650 1392240..1393973 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
HUU85_RS06655 1393979..1394689 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUU85_RS06660 1394714..1395610 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUU85_RS06665 1395722..1396243 + 522 WP_001055874.1 flavodoxin FldB -
HUU85_RS06670 1396283..1396690 - 408 WP_000244777.1 toxin CptA Toxin
HUU85_RS06675 1396671..1396937 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUU85_RS06680 1397180..1398160 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUU85_RS06685 1398356..1399015 - 660 WP_000250274.1 hemolysin III family protein -
HUU85_RS06690 1399179..1399490 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUU85_RS06695 1399535..1400968 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HUU85_RS06700 1401025..1401768 - 744 WP_000951962.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T166447 WP_000244777.1 NZ_CP058302:c1396690-1396283 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T166447 NZ_CP074159:c3866849-3866439 [Enterobacter sp. JBIWA005]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTGTTACTGATGCCGTGGCCGTTAAGCTATACGCCGCTGTGGCTGCTGTTACTCTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGAATTAATGCCCGTCAGGGAGAGATCAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATCCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTATTGCAACAGACGA
CGCAGGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT166447 WP_000354046.1 NZ_CP058302:c1396937-1396671 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT166447 NZ_CP074159:c3867096-3866830 [Enterobacter sp. JBIWA005]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGAATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCAGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGATACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References