Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1258340..1258923 Replicon chromosome
Accession NZ_CP058302
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag HUU85_RS06035 Protein ID WP_000254738.1
Coordinates 1258340..1258675 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag HUU85_RS06040 Protein ID WP_000581941.1
Coordinates 1258675..1258923 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS06020 1254227..1255525 - 1299 WP_000036723.1 phosphopyruvate hydratase -
HUU85_RS06025 1255613..1257250 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HUU85_RS06030 1257478..1258269 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HUU85_RS06035 1258340..1258675 - 336 WP_000254738.1 endoribonuclease MazF Toxin
HUU85_RS06040 1258675..1258923 - 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HUU85_RS06045 1259001..1261235 - 2235 WP_000226815.1 GTP pyrophosphokinase -
HUU85_RS06050 1261283..1262584 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T166446 WP_000254738.1 NZ_CP058302:c1258675-1258340 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T166446 NZ_CP074159:3639793-3640092 [Enterobacter sp. JBIWA005]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATCCCTTCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGCTATTCCTCCATCCGGGTAAACAGGCAATACAGGCTCGTATTTTGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT166446 WP_000581941.1 NZ_CP058302:c1258923-1258675 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT166446 NZ_CP074159:3640103-3640450 [Enterobacter sp. JBIWA005]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAGCTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTGAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCTAACGCCAGGACGCAGGAGGAACTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAACCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References