Detailed information of TA system TA166427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 460242..460881
• Replicon: chromosome
• Accession: NZ_CP058277
• Organism: Mycobacterium marinum strain MMA1

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: HXW97_RS01955
• Protein ID: WP_012396162.1
• Coordinates: 460242..460688 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: B2HEJ8
• Locus tag: HXW97_RS01960
• Protein ID: WP_011738542.1
• Coordinates: 460693..460881 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXW97_RS01930	455417..456937	+	1521	WP_012396157.1	carotenoid oxygenase family protein	-
HXW97_RS01935	456954..457427	+	474	WP_012396158.1	VOC family protein	-
HXW97_RS01940	457433..457867	-	435	WP_202097685.1	hypothetical protein	-
HXW97_RS01945	457938..458711	-	774	WP_012396160.1	VOC family protein	-
HXW97_RS01950	458877..460130	+	1254	WP_202097686.1	hypothetical protein	-
HXW97_RS01955	460242..460688	-	447	WP_012396162.1	SRPBCC family protein	Toxin
HXW97_RS01960	460693..460881	-	189	WP_011738542.1	antitoxin	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15851.39 Da
• Isoelectric Point: 8.6574

>T166427 WP_012396162.1 NZ_CP058277:c460688-460242 [Mycobacterium marinum]
MAKLSGSIDVPLSPDEAWMHASDLSRFKEWLTIHRVWRSTLPETLEKGAVVESIVQVKGMHNRIKWTIVRYQPPEGMTLN
GDGVGGVKVKLMAKVAAGEEGSIVSFDVHLGGPALFGPIGMVVAAALRSDINASLRNFVTVFAPSAAG

Nucleotide

• Length: 447 bp

>T166427 NZ_CP074149:c1255366-1255055 [Enterobacter sp. JBIWA008]
ATGCACGTAGTCTCAAGAGCGCCTTTCGACACGGCCACCAGACAATTTCCTAATCAAGCACTCGCCCTGGGCGATCTTTA
CCGGGTGCTAAAGAAGGAACACTACCCGTCACCGGACGATATGAGAAAGCGATTCCCCAGCCTGGACAGGATGAAATACA
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATTTAAGAGTGATGTTTTTAGCTGATTTTGAACGAGGAAAGATC
TTCATCAAACACATCAGCACACACGCAGAGTATGACAAGCTCACGGAACTCTACCGGAGAACGAAAGAATGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6586.35 Da
• Isoelectric Point: 4.7205

>AT166427 WP_011738542.1 NZ_CP058277:c460881-460693 [Mycobacterium marinum]
MGILDKAKDLLAQNADKVETVIDKAGEFVDEKTEGKYSDTIHKVQEGAKKVAGPGAAGEQQN

Nucleotide

• Length: 189 bp

>AT166427 NZ_CP074149:c1255058-1254639 [Enterobacter sp. JBIWA008]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGATCTGACCAACGAGCTCCCTTTCCTGGGCTCAAGCCCGTCGCGACA
AGATTACGAGGATGCGCTAGCGCTGGTGGAGTATCTGGTCGAACACGAACCCGATAGCTCGCTGGTAGAGATGCTCACCG
CCAAAATCGATAAATACGAAAACGAGTCTCCGGAGTTTGCAGAATTTAACTCACGTGTTGCCAGCATTCCGTCAGGCGTT
GCGTTATTGCGAACGCTGATAGACCAGTACCAACTCACTCAGAGCGACTTTGAGAACGAGATTGGTAAAAAGTCTCTGGT
GAGTCGGATCCTTAACGGACAGCGTACGCTAACGCTGGATCATATGCGCGCGCTGGCGAAGCGTTTTGATTTGCCGGTGA
GTGCGTTTGTGGGGAATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7LB02