Detailed information of TA system TA166424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200587..201223
• Replicon: chromosome
• Accession: NZ_CP058262
• Organism: Priestia megaterium strain BIM B-1314D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: HW576_RS01135
• Protein ID: WP_013055004.1
• Coordinates: 200873..201223 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: HW576_RS01130
• Protein ID: WP_013055003.1
• Coordinates: 200587..200868 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW576_RS01105	195953..196924	+	972	WP_177563296.1	UV DNA damage repair endonuclease UvsE	-
HW576_RS01110	196933..197535	-	603	WP_013054999.1	rhomboid family intramembrane serine protease	-
HW576_RS01115	197600..197965	+	366	WP_013055000.1	holo-ACP synthase	-
HW576_RS01120	198024..199082	+	1059	WP_013055001.1	outer membrane lipoprotein carrier protein LolA	-
HW576_RS01125	199196..200386	+	1191	WP_013055002.1	alanine racemase	-
HW576_RS01130	200587..200868	+	282	WP_013055003.1	hypothetical protein	Antitoxin
HW576_RS01135	200873..201223	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HW576_RS01140	201381..202217	+	837	WP_013055005.1	STAS domain-containing protein	-
HW576_RS01145	202220..202576	+	357	WP_013055006.1	STAS domain-containing protein	-
HW576_RS01150	202580..202981	+	402	WP_013055007.1	anti-sigma regulatory factor	-
HW576_RS01155	202995..204005	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
HW576_RS01160	204065..204397	+	333	WP_013055009.1	anti-sigma factor antagonist	-
HW576_RS01165	204394..204879	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
HW576_RS01170	204845..205639	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T166424 WP_013055004.1 NZ_CP058262:200873-201223 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T166424 NZ_CP074149:c306467-306267 [Enterobacter sp. JBIWA008]
ATTTATCCTTGTGCTAACTCCTTGACCATTATCGCTTCTCGCCTCTTTCGGTTGGCAAATGGCCTGGCGGGCGACAGAAT
CTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTATGCGGTGGTGACAAAAGCAGCCAGGCCAGAGACATACTTA
TGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT166424 WP_013055003.1 NZ_CP058262:200587-200868 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT166424 NZ_CP074149:c306266-306051 [Enterobacter sp. JBIWA008]
ATGAGCATGCATAAATTAACATCCTACGATCCAGCAAACGCGCTGGGCGTCATCGCGCGCTCGAAAGGAATATCGACCAT
TTCACAGCAAACTGGCCTATCACGAGAGCAACTGTATCGATCGTTCAGTGATAAAGGAAACCCAACGCTTAAAACCACTC
TGGCGGTCATGAAAGCATTGGGTCTTGGGTTAACAATCAAACACGCTGGAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9