Detailed information of TA system TA166420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 379..944
• Replicon: plasmid pED5
• Accession: NZ_CP058254
• Organism: Enterobacter roggenkampii strain ED5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ED5_RS22130
• Protein ID: WP_115490251.1
• Coordinates: 379..717 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ED5_RS22135
• Protein ID: WP_115490252.1
• Coordinates: 714..944 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ED5_RS22130	379..717	-	339	WP_115490251.1	endoribonuclease MazF	Toxin
ED5_RS22135	714..944	-	231	WP_115490252.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ED5_RS22140	1262..1813	+	552	WP_152049191.1	helix-turn-helix domain-containing protein	-
ED5_RS22145	2144..2380	-	237	WP_126680819.1	hypothetical protein	-
ED5_RS22150	3364..3750	-	387	WP_152049190.1	helix-turn-helix domain-containing protein	-
ED5_RS22155	3750..3941	-	192	WP_152049189.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4242	4242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-103)

Antitoxin

• Antitoxin PemI-like (1-75)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 11951.65 Da
• Isoelectric Point: 6.9473

>T166420 WP_115490251.1 NZ_CP058254:c717-379 [Enterobacter roggenkampii]
VTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFAVVCPITNQQKGYPFEVSVDGAKKTTGVILADQVKS
LDWKARAARTVDSVSDETVTAVVGMVSKIIKA

Nucleotide

• Length: 339 bp

>T166420 NZ_CP074147:c5868419-5868078 [Serratia sp. JSRIV001]
ATGGATGCTATATTTGTTGAGTTAAGTATTTTTGAAGCGAAGCGTGCTGATTATCTTTCCGATGATGAATTCAAGACATT
CCAGCAGATGCTGCTGATTAATCCATTCTGCGGTGATGTTATCCAGCACACTGGCGGGTTAAGGAAGGTCAGATTTGCGG
ATCACCGTCGTGGAAAAGGTAAACGAGGTGGCTTGCGGGTGATCTATTATTGGTGGCTTGAAAAGTCTCACTTTTTGCTT
TTTGTCATTTACGACAAAGACAAAAAAGATGACCTTAGCCGCAACGAGCGCGAGATGCTGCGTAGGATGTTGGAAATTTT
AAAACAAGGGGGCACCTTATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8351.61 Da
• Isoelectric Point: 6.4380

>AT166420 WP_115490252.1 NZ_CP058254:c944-714 [Enterobacter roggenkampii]
MTTAIGKWGNGAALRIPQPFMKQLGLSIGDAVNFCVTERNLVITKAGPTLEELLGQCTAENRHHEYFCDSQGKEML

Nucleotide

• Length: 231 bp

>AT166420 NZ_CP074147:c5868081-5867767 [Serratia sp. JSRIV001]
ATGAGCGAACTCAGCCTTTTTGAAGAACTATCCCAGGGAATGAAAGACTGGAGTGATTACAATGAGGGTAAAATCACGCT
TAAAACCCACGCCGTATCACTGAAAAAAAACATATCTATGTCACCAGACGAGTTAAAAGCAATACGTGAAAAACTGCGTC
TTTCACAAGCCGTTTTTGCGGATTACTTGCATACCGGTGTCACTACTTATCAAAACTGGGAACAGGGCAGAGCCAAACCC
AACAAACAGGCACTTCTTTTGATCCGTATGGTTGAAAAAGACCCCATGACGCTGGATACGTTAGCTGCCTTGTAA