Detailed information of TA system TA166408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 587808..588384
• Replicon: chromosome
• Accession: NZ_CP058253
• Organism: Enterobacter roggenkampii strain ED5

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A656ULU9
• Locus tag: ED5_RS02755
• Protein ID: WP_025912099.1
• Coordinates: 587808..588095 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: ED5_RS02760
• Protein ID: WP_177317069.1
• Coordinates: 588082..588384 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ED5_RS02725	582821..582937	+	117	Protein_526	amino acid-binding protein	-
ED5_RS02730	582930..583625	+	696	WP_177317068.1	winged helix-turn-helix domain-containing protein	-
ED5_RS02735	583771..584241	+	471	WP_008501325.1	MarR family transcriptional regulator	-
ED5_RS02740	584238..585305	+	1068	WP_025912094.1	HlyD family secretion protein	-
ED5_RS02745	585295..586371	+	1077	WP_045418349.1	DUF2955 domain-containing protein	-
ED5_RS02750	586368..587639	-	1272	WP_126348752.1	DUF445 domain-containing protein	-
ED5_RS02755	587808..588095	+	288	WP_025912099.1	BrnT family toxin	Toxin
ED5_RS02760	588082..588384	+	303	WP_177317069.1	BrnA antitoxin family protein	Antitoxin
ED5_RS02765	588415..589053	-	639	WP_177317070.1	LysE family translocator	-
ED5_RS02770	589101..589844	-	744	WP_161622971.1	AraC family transcriptional regulator	-
ED5_RS02775	589996..591366	+	1371	WP_058654250.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
ED5_RS02780	591412..591732	-	321	WP_177317071.1	hypothetical protein	-
ED5_RS02785	591732..592277	-	546	WP_008501313.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11235.68 Da
• Isoelectric Point: 7.4007

>T166408 WP_025912099.1 NZ_CP058253:587808-588095 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T166408 NZ_CP074147:3459245-3459580 [Serratia sp. JSRIV001]
ATGAAGACCATCAACAAACTCCCCCACACCCCGACCCATGCCAATCCCACCTTTATCGAATACCAGCAACTGTTGGCCTG
GTTGCTGGGCCATTGCTATGGCCTGGAACTTAACGATACGCCCTATCACGACGACCAGGCGATTGCTCAACAGATTGAAC
ATGGCATTACCGTAAGGGAGACCGTCAACGAACTGGTGGAGAAATTTGATCTGGTACGCATTGATCGCCCCGGTTTCAGC
CTGATGGCACAAGACCCAACCCTAAGCGGGGGCGATCTCCTACGGGCCCGCAGTGCGCTAGGTTTGCGAAGCCCAGCCAT
TCGCCGCCTTGCCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11431.08 Da
• Isoelectric Point: 10.2483

>AT166408 WP_177317069.1 NZ_CP058253:588082-588384 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVVEFKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT166408 NZ_CP074147:3459659-3460027 [Serratia sp. JSRIV001]
ATGAAAGATAGCGATACCGATAACCACGAAGACAGCACACCGTCCCCCATCTGGGGCCTGCACTGTGCTATCACGCCAAG
ATTAGGTGCCAGACTGGTGCAACAGGGCAATCGTCTGCATTTTCTGGTTGACCGCGCCGGTCTGGTTGGCACCTTTTCAC
CCAACGACCTCAAGGGGCTGAATAGCGCCTTCCCCGTGCTGATTGAACAGTTGGAAACCTGTCTGCGCTCGGGTGAACTC
GACCAGCGCCGCCAGCACTGCGTCACGGTCCAGCACGCCGGTTTTACCTGCCAGGCTGATACGTTGGGCAGTTTTGGGCA
TGTCTATATCGCTGTTTATCAGACAGCACAGCACACGAGCGGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A656ULU9