Detailed information of TA system TA166406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 149525..150132
• Replicon: chromosome
• Accession: NZ_CP058253
• Organism: Enterobacter roggenkampii strain ED5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ED5_RS00695
• Protein ID: WP_177316983.1
• Coordinates: 149525..149710 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A155E9Y3
• Locus tag: ED5_RS00700
• Protein ID: WP_032675741.1
• Coordinates: 149725..150132 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ED5_RS00680	145957..147495	+	1539	WP_023345218.1	aldehyde dehydrogenase AldB	-
ED5_RS00685	147492..148454	-	963	WP_177316981.1	LysR family transcriptional regulator	-
ED5_RS00690	148575..149315	+	741	WP_177316982.1	MipA/OmpV family protein	-
ED5_RS00695	149525..149710	+	186	WP_177316983.1	type II toxin-antitoxin system HicA family toxin	Toxin
ED5_RS00700	149725..150132	+	408	WP_032675741.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ED5_RS00705	150136..151212	-	1077	WP_032654523.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
ED5_RS00710	151558..152253	-	696	WP_177316984.1	L-ribulose-5-phosphate 4-epimerase	-
ED5_RS00715	152247..153107	-	861	WP_177316985.1	L-ribulose-5-phosphate 3-epimerase	-
ED5_RS00720	153110..153763	-	654	WP_177316986.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6860.01 Da
• Isoelectric Point: 11.0014

>T166406 WP_177316983.1 NZ_CP058253:149525-149710 [Enterobacter roggenkampii]
VKSADVITVLISHGWKCVRTKGSHHQFRHPEQKGLVTVPHPKKDIKPGTLAQIWRQAGIKD

Nucleotide

• Length: 186 bp

>T166406 NZ_CP074147:1500349-1500663 [Serratia sp. JSRIV001]
ATTTCATCTGTACCGGCCTCGGTGCCGGTTTCGTCACGCCCGTCGCCCGTACAGGTCTGGCAGCAACTGTTAACGTATCT
GCTGGAGCACCATTACGGCCTCACACTCAACGACACACCATTCCATGATGACACGGCCATCCAGGAACATATCGAGGCAG
GGATCACGCTTGCCGATGCAGTGAATTTCCTGGTGAATCGCTATGAACTGGTGCGCACTGATCGTAAGGGTTTCAGTTGG
CAGGAGCAGACGCCATTCCTGACCGCCACCGATATTCTCAGAGCAAGGCGTGCTACCGGGCTGATGAATACGTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15010.78 Da
• Isoelectric Point: 4.3897

>AT166406 WP_032675741.1 NZ_CP058253:149725-150132 [Enterobacter roggenkampii]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDNALPLPGNVEAHLESRPEDFTGGQWL
LVDINMKQFDGRAERINITMPRRLLVKIDSFVSEHPQFGNRSAFLAEAARRVLPG

Nucleotide

• Length: 408 bp

>AT166406 NZ_CP074147:1499983-1500306 [Serratia sp. JSRIV001]
ATGCCATTAACACCACCTCATGAATGGGGGTTACAAAGCGATATCACCCCACAATTTGGCGCAAGATTGGTTCAGGAAGG
TAACCGGCTGCATTACCTGACTGACAGGGCTGGGTTGACGGGGAGCTTCAGCCCGGAGCAATTACAGAAACTGGATCAGG
CTTTCCCGCTGTTTATAGAACAACTGGAAAATTTGCTGCGCTCCGGCGAGCTAAATCCACGTCAGCAACATCAGGTGACC
ATTCAGCTTGCGAGTTTAACCTGCATCGCTGATACCCGTGGTAGCTGCGGCTACGTTTACATTTCTATTTATCCAACCCA
GTAA

Structures

Antitoxin

• AlphaFold DB: A0A155E9Y3