Detailed information of TA system TA166379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518655..519291
• Replicon: chromosome
• Accession: NZ_CP058242
• Organism: Bacillus subtilis strain SP1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HWV68_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 518941..519291 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: HWV68_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 518655..518936 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWV68_RS02600	515014..515613	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
HWV68_RS02605	515708..516073	+	366	WP_003234281.1	holo-ACP synthase	-
HWV68_RS02610	516239..517255	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
HWV68_RS02615	517370..518539	+	1170	WP_003234284.1	alanine racemase	-
HWV68_RS02620	518655..518936	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HWV68_RS02625	518941..519291	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HWV68_RS02630	519406..520230	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
HWV68_RS02635	520235..520600	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
HWV68_RS02640	520604..521005	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
HWV68_RS02645	521017..522024	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
HWV68_RS02650	522086..522415	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
HWV68_RS02655	522412..522894	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
HWV68_RS02660	522860..523648	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
HWV68_RS02665	523648..524247	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T166379 WP_003156187.1 NZ_CP058242:518941-519291 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T166379 NZ_CP074143:261977-262327 [Serratia sp. JSRIV002]
TTGTGGACTGTTATTTTTACCTCGACATTTGATTCTTGGTTGCAAGAGCAGGAAGAAGGGATGCAGGAGAAGGTACTGGC
TGACCTGATGAACCTGGAGACGTATGGGCCTCAACTGCCACGTCCTTACGCTGACACGGTAAAAGGTTCAAGATACAAGA
ATATGAAAGAGCTGCGTGTTCAGTATTCGGGCCGCCCAATCCGTGCCTTTTTTGTTTTCGACCCCAAACGCCAAGCCATT
GTGTTGTGCGCTGGTGATAAAAGTAATGATAAGCGTTTTTACGAGACAATGATCCGAATAGCGGATGATGAATTTACGGC
GTATTTAGCCACACAAGAGGTATCCAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT166379 WP_003225183.1 NZ_CP058242:518655-518936 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT166379 NZ_CP074143:262324-262623 [Serratia sp. JSRIV002]
ATGAAAACGTTACGTGATGCAGTCGCTGCCCGTTCTCTTGAGAGCCAAGCCCGTATCAAGGAAATGGCCGACGAAATGAT
GCTAGAAACAGGTTTGCAACTGATGCGTGATGAGCTGCAACTGTCACAGAAAAGCCTGGCCGAAGCTATGGGCGTAAGCC
AATCCGCGGTTGCACAAATAGAGCAGCGTGGTAATGACGTAAAGTTAGCGACGCTAAAACGCTATATTGAAGCTATGGGT
GGGAAGTTGAGTCTGACTGTAGAGATGCCGGAAGGCGGTGGGCGAGTCTTCCATATCTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32