Detailed information of TA system TA166378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3855177..3855862
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: HWS75_RS18130
• Protein ID: WP_003417998.1
• Coordinates: 3855452..3855862 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HWS75_RS18125
• Protein ID: WP_003912220.1
• Coordinates: 3855177..3855455 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS18105	3851166..3852767	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
HWS75_RS18110	3852784..3853488	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
HWS75_RS18115	3853606..3854172	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
HWS75_RS18120	3854234..3855121	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
HWS75_RS18125	3855177..3855455	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HWS75_RS18130	3855452..3855862	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWS75_RS18135	3855895..3857631	-	1737	WP_003418002.1	cholesterol oxidase	-
HWS75_RS18140	3857687..3858814	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
HWS75_RS18145	3858834..3860423	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T166378 WP_003417998.1 NZ_CP058236:3855452-3855862 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T166378 NZ_CP074143:c244584-244102 [Serratia sp. JSRIV002]
ATGGGAATGAAAGCTCCCGAGCCCTTGTCATCCGGGCATAACCTCGCTGACTTTTGCAGTTCAGAGCCGATGCTTAATGA
GTGGCTGAGAAAGAAAGCGCTTAAAAATCATAGCGCAGGAATTTCCCGCGTTTATGTTATTTGCACAGAGGAAACCCATC
GGGTTATTGGATACTATTGCCTAGCAACGGGTAGCGTTCAGAGGAATACCGCCCCCGGAGCTTACAGACGCAATGCGCCA
GAGTCACTTCCGGTTATCGTTCTTGGCAGACTTGCTATCGATCAGGCGTACTCAGGCAAGGGCCTAGGGGTCGCATTGTT
AAAAGACGCGATATTTCGGACAAAGAATATTGCTCAACAAGTTGGCGTGAGAGCGTTAATCGTTCATGCATTGAATGAAG
GGGTGCGAAACTTCTACATCAAGTTCGCCTTCTCCCCCTCCCCTGAACACCCACTGATGCTGTTTTACCCAATAAAGCTT
TAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT166378 WP_003912220.1 NZ_CP058236:3855177-3855455 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT166378 NZ_CP074143:c244853-244575 [Serratia sp. JSRIV002]
ATGAAACCTGAAACTAGAGAAGCCCCGATCAATATTCGGGCAAAAGCCTCTCAACGCGACCTGATCGACATGGCTGCAAG
TCTCTTGTCGAAGTCGCGTACCGATTTCATGCTTGAAGTGGCTTGCCGCGAGGCGCAGGACATCCTACTGGACCAGCGTT
TGTTTATCTTAGACGACGAACAATATGATGCGTTTCTGACCGAACTGGATGCGCCTATCACCGCTGAACGCCAGGCCCGT
ATTAACGACCTGATGAGCCGCAAATCGCCATGGGAATGA

Structures

Toxin

• AlphaFold DB: G0TIR9