Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3132185..3132755 Replicon chromosome
Accession NZ_CP058236
Organism Mycobacterium tuberculosis strain 060827

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag HWS75_RS14900 Protein ID WP_003414166.1
Coordinates 3132185..3132541 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag HWS75_RS14905 Protein ID WP_003901465.1
Coordinates 3132525..3132755 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWS75_RS14880 3127637..3129325 - 1689 Protein_2937 alpha/beta hydrolase family protein -
HWS75_RS14885 3129329..3129655 - 327 WP_003414157.1 hypothetical protein -
HWS75_RS14890 3129828..3130415 + 588 WP_031667424.1 DUF3558 family protein -
HWS75_RS14895 3130434..3132083 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
HWS75_RS14900 3132185..3132541 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
HWS75_RS14905 3132525..3132755 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
HWS75_RS14910 3132798..3133841 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
HWS75_RS14915 3133840..3134307 + 468 WP_003414177.1 DUF1778 domain-containing protein -
HWS75_RS14920 3134483..3134737 - 255 WP_003917684.1 hypothetical protein -
HWS75_RS14925 3134885..3135289 + 405 WP_003414181.1 hypothetical protein -
HWS75_RS14930 3135286..3135477 + 192 WP_003414184.1 hypothetical protein -
HWS75_RS14935 3135694..3135954 + 261 Protein_2948 transposase -
HWS75_RS14940 3137064..3137321 + 258 WP_003899489.1 hypothetical protein -
HWS75_RS14945 3137426..3137737 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T166369 WP_003414166.1 NZ_CP058236:c3132541-3132185 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T166369 NZ_CP074140:c4800324-4799902 [Serratia sp. JSRIV004]
GTGGCCCTGTGGCGATGTGATGTCCGCGTTTCCTGGCGTACCCAGTTAGTCTCGTTGCTGACTCACGGCCTGCTGATTTT
ACTGATCCTGATCGCCCCGTGGCCGGAAGGCTATGGCCCGATCTGGTTGGTGCTGTTGACGTTGGTGGTGTTTGAGTGCA
TTCGCAGCCAAAAGCGCATTGCATCCAGGCAGGGAGAACTGCGGTTGCTGGCTGAACGCCGGATTGAGTGGCATGGCCGA
GAATGGCTGATGGTGAACAAGCCCTGGATGCCTGGGTTCGGTATTTTGCTGTCGCTTCAGCAGATCGACGGCAAGAAACG
CCATCGGCTGTGGTTGGCTTCGGATTCCATGAGCAAAGCGGATTGGCGCCAACTGCGTCAACGGTTGTTGTTTGAACAAT
CCGCTACCGATGAGGACGTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT166369 WP_003901465.1 NZ_CP058236:c3132755-3132525 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT166369 NZ_CP074140:c4800571-4800305 [Serratia sp. JSRIV004]
ATGGATATTAATAATAAAGCACGTATTCACTGGGCCTGCCGCCGCGGCATGCGCGAGTTGGATATCTCGATCATGCCGTT
TTTCGAACATGAATACGATACGCTGAGTGATGCAGACAAGGCCGTATTCATCCGCTTGCTCGAATGTGACGACCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCTCCGCAGGATCAAGATCTGCAGAGAATGGTGTCCCTGATCCAAATGCGAAAT
AAAGCCCGTGGCCCTGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References