Detailed information of TA system TA166367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3092200..3092879
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: HWS75_RS14670
• Protein ID: WP_003414059.1
• Coordinates: 3092200..3092616 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: HWS75_RS14675
• Protein ID: WP_003414061.1
• Coordinates: 3092613..3092879 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS14645	3088252..3089154	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HWS75_RS14650	3089223..3089975	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
HWS75_RS14660	3090219..3090494	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
HWS75_RS14665	3090491..3092113	-	1623	WP_003909739.1	class I SAM-dependent DNA methyltransferase	-
HWS75_RS14670	3092200..3092616	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
HWS75_RS14675	3092613..3092879	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HWS75_RS14680	3092905..3093300	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
HWS75_RS14685	3093297..3093566	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
HWS75_RS14690	3093576..3094670	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
HWS75_RS14695	3094667..3095086	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
HWS75_RS14700	3095085..3095159	+	75	Protein_2901	hypothetical protein	-
HWS75_RS14705	3095160..3095639	-	480	WP_003414073.1	dihydrofolate reductase	-
HWS75_RS14710	3095710..3096510	-	801	WP_003911953.1	thymidylate synthase	-
HWS75_RS14715	3096666..3097403	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T166367 WP_003414059.1 NZ_CP058236:c3092616-3092200 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T166367 NZ_CP074140:c4375144-4374755 [Serratia sp. JSRIV004]
ATGTATATGTTCGACACTAACACGGTGAGCCAGCTTTTTCGTCAACATCCCCGGCTGCTTGCGGCTATGGGCCGGGTACC
GCCATCGTCGGTGTGTATTTCCAGCATAACCCAGGCGGAACTGCTTTATGGTGTCGCCAAGCGGCAGAACAAGGCGCTAA
AACAGACGGTGATGATGTTCCTGGACGCCGTCACCGTTTACTCCTGGGACTCTGCGGCGGCGCATTGCTATGGTGTCATG
CGCGCAAGTATGGAAAAACAAGGCAGACCGATGGGGGCGTTGGATCAGCTTATTGCCGCACATGCGGTTAGCCGTGGCGC
AACCATAGTGACCAGCGATCGGGCTTTCACTATGGTCGCGGAGCTTGAGGTGGAAGACTGGACGCTGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT166367 WP_003414061.1 NZ_CP058236:c3092879-3092613 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT166367 NZ_CP074140:c4375411-4375148 [Serratia sp. JSRIV004]
ATGGAAAGAGTCGCCAAGCTTTTTAAAAACGGTAGAAATCAGGCGGTGAGATTGCCAGTGGAATTCGAATTCGATGTCGA
TCAGGTTTATATTCGCCGGGATGAACAGGGTAACGTTATCTTGTCGAAGCATCCAATGAAATTGGACTCCTGGCAACCGC
TACTGACCATGCTGCAAAACGTGGCGGTTCCTGCTGACTTCCTGACTGCTGACGATCGCCAGCAAGGGGTAGCAGAACGG
GATCCGTTCAAGGAGGAACAGTAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64