Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2892416..2893053 Replicon chromosome
Accession NZ_CP058236
Organism Mycobacterium tuberculosis strain 060827

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag HWS75_RS13580 Protein ID WP_003413180.1
Coordinates 2892416..2892811 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag HWS75_RS13585 Protein ID WP_003413183.1
Coordinates 2892808..2893053 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWS75_RS13540 2888501..2889160 + 660 WP_003900846.1 LppA family lipoprotein -
HWS75_RS13545 2889157..2889816 + 660 WP_003900847.1 LppA family lipoprotein -
HWS75_RS13550 2889813..2890475 + 663 WP_003900848.1 LppA family lipoprotein -
HWS75_RS13555 2890472..2890750 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
HWS75_RS13560 2890843..2891256 + 414 WP_003413164.1 PIN domain nuclease -
HWS75_RS13565 2891295..2891552 + 258 WP_003909690.1 CopG family transcriptional regulator -
HWS75_RS13570 2891549..2891926 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
HWS75_RS13575 2891942..2892316 - 375 WP_003413177.1 hypothetical protein -
HWS75_RS13580 2892416..2892811 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
HWS75_RS13585 2892808..2893053 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
HWS75_RS13590 2893464..2893883 - 420 WP_003413190.1 A24 family peptidase -
HWS75_RS13595 2893895..2894704 - 810 WP_003413193.1 shikimate dehydrogenase -
HWS75_RS13600 2894701..2895954 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
HWS75_RS13605 2895947..2896459 - 513 WP_235732996.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T166364 WP_003413180.1 NZ_CP058236:c2892811-2892416 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T166364 NZ_CP074140:c1650663-1650460 [Serratia sp. JSRIV004]
ATGGAAAGTGGAGAACTAATCAAACGATTGCAGGCTGACGGATGGATTATCAGGAAGTCGAGGGGCAAGAAGGCAACAGG
AAGCCACGTAACAATGACAAAACCGGGGGTAGCAAAAATCATCACCATCCCCCACCCCAAAAAAGATTCTTCAAAGGGCA
TATTGAGGCAAGTACAACAGATATCCGGCCTCAAATTGATTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT166364 WP_003413183.1 NZ_CP058236:c2893053-2892808 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT166364 NZ_CP074140:c1650422-1650015 [Serratia sp. JSRIV004]
ATGATTTATCCAATATTCATCTTTAAGACGTCAGAAGGCTTTGATGGCTACTTTCCCGATGTAGAAGGCTGTTTTTTTGC
TGGTGAGAAAATGTCAGATATCCCAAAAAATGCAGAAGAGGCCTTTGGGGTACATATGGAAGCACTCATCGAAGAGGGGT
TCCCTGTTCCAGCCGCCCCCCAAGATCCGCAATCCTATCTCAATGATAGTCGGCTGCGAGAAGACGGCGGCATTTTGGGA
TTTGTTGAGATAGATCCGGCCAAGTATGAGGATAAATCGATTAAGTTTAATCTGACGATGCCACGTAACTTACTAACGGC
AATCGATAAGTACATAGAAACCACACGCGGCTACAAAAACCGCAGCCAGTTTCTAGCGGAATTAGCCCGCGAAAAAATAT
CCCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References