Detailed information of TA system TA166363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 2891295..2891926
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: HWS75_RS13570
• Protein ID: WP_003413174.1
• Coordinates: 2891549..2891926 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7U8Y8P4
• Locus tag: HWS75_RS13565
• Protein ID: WP_003909690.1
• Coordinates: 2891295..2891552 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS13530	2886553..2886867	+	315	WP_009937839.1	hypothetical protein	-
HWS75_RS13535	2887163..2888374	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
HWS75_RS13540	2888501..2889160	+	660	WP_003900846.1	LppA family lipoprotein	-
HWS75_RS13545	2889157..2889816	+	660	WP_003900847.1	LppA family lipoprotein	-
HWS75_RS13550	2889813..2890475	+	663	WP_003900848.1	LppA family lipoprotein	-
HWS75_RS13555	2890472..2890750	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
HWS75_RS13560	2890843..2891256	+	414	WP_003413164.1	PIN domain nuclease	-
HWS75_RS13565	2891295..2891552	+	258	WP_003909690.1	CopG family transcriptional regulator	Antitoxin
HWS75_RS13570	2891549..2891926	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWS75_RS13575	2891942..2892316	-	375	WP_003413177.1	hypothetical protein	-
HWS75_RS13580	2892416..2892811	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
HWS75_RS13585	2892808..2893053	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
HWS75_RS13590	2893464..2893883	-	420	WP_003413190.1	A24 family peptidase	-
HWS75_RS13595	2893895..2894704	-	810	WP_003413193.1	shikimate dehydrogenase	-
HWS75_RS13600	2894701..2895954	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
HWS75_RS13605	2895947..2896459	-	513	WP_235732996.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T166363 WP_003413174.1 NZ_CP058236:2891549-2891926 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T166363 NZ_CP074140:c1572379-1572176 [Serratia sp. JSRIV004]
ATGACAAAAATTGACTATCTGATGCGTTTGCGTAAATGCACCACGATTGAAACCCTTGAGCGCGTGATCGAGAAAAATAA
GTACGAACTTTCTGACGATGAGCTAGAGCTGTTCTACTCTGCCGCCGATCATCGCCTGGCCGAACTGACCATGAACAAGC
TATACGATAAAATTCCGGTCGCCGTTTGGAAATTTGTAAGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9511.67 Da
• Isoelectric Point: 10.2895

>AT166363 WP_003909690.1 NZ_CP058236:2891295-2891552 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRDSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT166363 NZ_CP074140:c1572798-1572430 [Serratia sp. JSRIV004]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAACTCAAATTTTTGTGTGAAAATTTGTACGACGAAGGCATCGC
TACCCTGGGTGACAGCCATCACGGCTGGGTAAACGATCCAACGTCCGCCGTCAATCTGCAGCTTAATGAGCTGATCGAAC
ATATTGCTTCGTTTGTAATGAGTTTTAAAATTAAATACATGGACGAAGGTGATTTATCGGAACTGGTCGAAGAGTACCTC
GACGATACCTACACGCTGTTTAGCAGCTACGGTATTAATGACTCCGATTTGCGTCGCTGGCAAAAAACCAAGGCACGCAC
ATTCAGATTGTTCTCAGGAGAGGGCGTCTGTACCACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A7U8Y8P4