Detailed information of TA system TA166361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2876303..2876943
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: HWS75_RS13470
• Protein ID: WP_003412970.1
• Coordinates: 2876303..2876722 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: HWS75_RS13475
• Protein ID: WP_003412975.1
• Coordinates: 2876719..2876943 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS13440	2871889..2872611	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
HWS75_RS13445	2873128..2873355	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
HWS75_RS13450	2873352..2873753	+	402	WP_003412963.1	PIN domain-containing protein	-
HWS75_RS13455	2873788..2874708	-	921	WP_003412965.1	restriction endonuclease	-
HWS75_RS13460	2875049..2875255	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HWS75_RS13465	2875353..2876302	+	950	Protein_2659	ERCC4 domain-containing protein	-
HWS75_RS13470	2876303..2876722	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWS75_RS13475	2876719..2876943	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
HWS75_RS13480	2876974..2879813	-	2840	Protein_2662	amino acid decarboxylase	-
HWS75_RS13485	2879885..2880286	-	402	WP_003412981.1	hypothetical protein	-
HWS75_RS13490	2880286..2880756	-	471	WP_003412985.1	transcription antitermination factor NusB	-
HWS75_RS13495	2880759..2881322	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T166361 WP_003412970.1 NZ_CP058236:c2876722-2876303 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T166361 NZ_CP074140:c794231-793914 [Serratia sp. JSRIV004]
ATGAAACAATTTTCTGTTTATAAAAATCAATCTAAAAACAAAAAAGCCTATCCTTTCTTTATCGTTGTTCAGTCCGATTT
ACTCGATCACCTTAGCACTCGTTTGGTCATGCCCTTGGCTTTAAAGCATGCAACAAACTCACAGGTCAAAGCACTGACTC
CTGTTTTGTCTGTGGATCAACAAGATTACGTGATCCTTACTAGTTTACTGACGACTACGGATGTCAAAAATCTTAATGCA
GATTGCCTAGTTACCAATGCAGCCCATCTGCGTGATGAGCTGGTTGCGGCCATCGACCTGTTAGTTTTGGGCATTTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT166361 WP_003412975.1 NZ_CP058236:c2876943-2876719 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT166361 NZ_CP074140:c794476-794231 [Serratia sp. JSRIV004]
ATGGGAACCGTTTCAGTAATAAAAAAGCGCAATACTAACGTGTACTTAAGTGTCGACCTGATCGAAAAGGCTAAAGCATT
AGACCTAAACTTATCGGCTACACTAAACCACGCATTAGAACTCGCAGTGAAGAAGGGGCAACGTGAACGTTGGTTAGCGG
AAAATCGTGCTGGTCTTGAAGCATTGAATGGCTTCGTTGAGGAGAACGGCCTTTTTTCTGATGATGCTGACTTTGGTGTC
CTGTAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09