Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2347403..2348039 Replicon chromosome
Accession NZ_CP058236
Organism Mycobacterium tuberculosis strain 060827

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag HWS75_RS10980 Protein ID WP_003410654.1
Coordinates 2347629..2348039 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag HWS75_RS10975 Protein ID WP_003410651.1
Coordinates 2347403..2347636 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWS75_RS10960 2342851..2343252 + 402 WP_009937711.1 metal ABC transporter permease -
HWS75_RS10965 2343253..2343657 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
HWS75_RS10970 2343741..2347325 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
HWS75_RS10975 2347403..2347636 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
HWS75_RS10980 2347629..2348039 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
HWS75_RS10985 2348023..2349114 + 1092 WP_003900454.1 precorrin-3B synthase -
HWS75_RS10990 2349124..2349750 + 627 WP_003410658.1 precorrin-8X methylmutase -
HWS75_RS10995 2349747..2351273 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
HWS75_RS11000 2351219..2352442 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T166357 WP_003410654.1 NZ_CP058236:2347629-2348039 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T166357 NZ_CP074140:241408-241758 [Serratia sp. JSRIV004]
TTGTGGACTGTTATTTTTACCTCGACATTTGATTCTTGGTTGCAAGAGCAGGAAGAAGGGATGCAGGAGAAGGTACTGGC
TGACCTGATGAACCTGGAGACGTATGGGCCTCAACTGCCACGTCCTTACGCTGACACGGTAAAAGGTTCAAGATACAAGA
ATATGAAAGAGCTGCGTGTTCAGTATTCGGGCCGCCCAATCCGTGCCTTTTTTGTTTTCGACCCCAAACGCCAAGCCATT
GTGTTGTGCGCTGGTGATAAAAGTAATGATAAGCGTTTTTACGAGACAATGATCCGAATAGCGGATGATGAATTTACGGC
GTATTTAGCCACACAAGAGGTATCCAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT166357 WP_003410651.1 NZ_CP058236:2347403-2347636 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT166357 NZ_CP074140:241755-242054 [Serratia sp. JSRIV004]
ATGAAAACGTTACGTGATGCAGTCGCTGCCCGTTCTCTTGAGAGCCAAGCCCGTATCAAGGAAATGGCCGACGAAATGAT
GCTAGAAACAGGTTTGCAACTGATGCGTGATGAGCTGCAACTGTCACAGAAAAGCCTGGCCGAAGCTATGGGCGTAAGCC
AATCCGCGGTTGCACAAATAGAGCAGCGTGGTAATGACGTAAAGTTAGCGACGCTAAAACGCTATATTGAAGCTATGGGT
GGGAAGTTGAGTCTGACTGTAGAGATGCCGGAAGGCGGTGGGCGAGTCTTCCATATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References