Detailed information of TA system TA166355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2278257..2278883
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: HWS75_RS10685
• Protein ID: WP_003410075.1
• Coordinates: 2278485..2278883 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: HWS75_RS10680
• Protein ID: WP_003911750.1
• Coordinates: 2278257..2278484 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS10670	2276296..2276640	-	345	WP_003410065.1	ferredoxin family protein	-
HWS75_RS10675	2276829..2277998	-	1170	WP_003899126.1	ATP-binding protein	-
HWS75_RS10680	2278257..2278484	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HWS75_RS10685	2278485..2278883	+	399	WP_003410075.1	PIN domain nuclease	Toxin
HWS75_RS10690	2279066..2279497	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
HWS75_RS10695	2279598..2280038	+	441	WP_235732985.1	DUF1398 domain-containing protein	-
HWS75_RS10700	2280475..2280654	-	180	Protein_2114	hypothetical protein	-
HWS75_RS10705	2280742..2281905	+	1164	WP_235732986.1	IS110 family transposase	-
HWS75_RS10710	2282033..2283289	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T166355 WP_003410075.1 NZ_CP058236:2278485-2278883 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T166355 NZ_CP074140:132267-132602 [Serratia sp. JSRIV004]
ATGAAGGCCATCGACAAAATTCCCCATACCGCACCTAACGCCAATCAGGCGTTAGCCGAATACGAGCAATTGCTTACCTG
GTTGCTGAACCACTGTTATGGATTAGAACTCAACGATACGCCGTATCACGACCCACAAGCTATCGCTCAACAGATTGAGT
ACGGAATGACAGTACGGGAAACCATCAACGAGCTGGTCGAGAAATTCGACCTGGTACGTATTGACCGCCCCGGTTTCAGT
CTGCTGGCACAGGACCCTACGCTGAGTGGTGGGGATATGCTACTCGCCCGTAGTGCGTTAGGGCTGCGAAACCCCGTAAA
TCGCCGCCTCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT166355 WP_003911750.1 NZ_CP058236:2278257-2278484 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT166355 NZ_CP074140:132679-132963 [Serratia sp. JSRIV004]
ATGAAAAATCTCGAACCTGATATCCCACCCACGGCTCCGGTCTGGGGTCTGCAATGTGCTATTACGCCACGTCTGGGGGC
CAGACTGGTTCAACAGGGTCGCCACCTGCATTTTCTGGCTGACCGCGCCGGTTTGATTGGCACGTTCACACCCGAAGCCA
TCAAGGCTCTCGATGTGGCTTTTCCGGTGTTGATTGAACAGTTGGAGAACTATCTGCGATCTGGCGAGCTCGATCCACGT
CGCCAGCATTGTGTCACTGTTCAGCTGTCACCCAGTACAGAGTGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8