Detailed information of TA system TA166349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2219616..2220319
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: HWS75_RS10320
• Protein ID: WP_003409778.1
• Coordinates: 2219616..2219945 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: HWS75_RS10325
• Protein ID: WP_003409780.1
• Coordinates: 2219942..2220319 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS10300	2216000..2217069	+	1070	Protein_2034	epoxide hydrolase EphB	-
HWS75_RS10305	2217066..2217581	+	516	WP_003409718.1	flavin reductase family protein	-
HWS75_RS10310	2217578..2218639	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
HWS75_RS10315	2218636..2219406	+	771	WP_003409775.1	SDR family oxidoreductase	-
HWS75_RS10320	2219616..2219945	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HWS75_RS10325	2219942..2220319	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
HWS75_RS10330	2220316..2220906	-	591	WP_003909548.1	SEC-C metal-binding domain-containing protein	-
HWS75_RS10335	2220961..2222325	+	1365	WP_235732983.1	HNH endonuclease signature motif containing protein	-
HWS75_RS10340	2222480..2222932	-	453	WP_003899095.1	lipoprotein	-
HWS75_RS10345	2222996..2223397	+	402	WP_003409869.1	hypothetical protein	-
HWS75_RS10350	2223390..2223572	-	183	WP_003409870.1	hypothetical protein	-
HWS75_RS10355	2223686..2224036	-	351	WP_003409871.1	hypothetical protein	-
HWS75_RS10360	2224047..2224949	-	903	WP_003409874.1	hypothetical protein	-
HWS75_RS10365	2224970..2225161	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T166349 WP_003409778.1 NZ_CP058236:c2219945-2219616 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T166349 NZ_CP074137:c4572546-4572124 [Serratia sp. JSRIV006]
GTGGCCCTGTGGCGATGTGATGTCCGCGTTTCCTGGCGTACCCAGTTAGTCTCGTTGCTGACTCACGGCCTGCTGATTTT
ACTGATCCTGATCGCCCCGTGGCCGGAAGGCTATGGCCCGATCTGGTTGGTGCTGTTGACGTTGGTGGTGTTTGAGTGCA
TTCGCAGCCAAAAGCGCATTGCATCCAGGCAGGGAGAACTGCGGTTGCTGGCTGAACGCCGGATTGAGTGGCATGGCCGA
GAATGGCTGATGGTGAACAAGCCCTGGATGCCTGGGTTCGGTATTTTGCTGTCGCTTCAGCAGATCGACGGCAAGAAACG
CCATCGGCTGTGGTTGGCTTCGGATTCCATGAGCAAAGCGGATTGGCGCCAACTGCGTCAACGGTTGTTGTTTGAACAAT
CCGCTACCGATGAGGACGTATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT166349 WP_003409780.1 NZ_CP058236:c2220319-2219942 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT166349 NZ_CP074137:c4572793-4572527 [Serratia sp. JSRIV006]
ATGGATATTAATAATAAAGCACGTATTCACTGGGCCTGCCGCCGCGGCATGCGCGAGTTGGATATCTCGATCATGCCGTT
TTTCGAACATGAATACGATACGCTGAGTGATGCAGACAAGGCCGTATTCATCCGCTTGCTCGAATGTGACGACCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCTCCGCAGGATCAAGATCTGCAGAGAATGGTGTCCCTGATCCAAATGCGAAAT
AAAGCCCGTGGCCCTGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8