Detailed information of TA system TA166346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1780210..1780823
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: HWS75_RS08355
• Protein ID: WP_003407786.1
• Coordinates: 1780419..1780823 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: HWS75_RS08350
• Protein ID: WP_009935474.1
• Coordinates: 1780210..1780413 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS08335	1775474..1778377	+	2904	WP_003904644.1	MMPL family transporter	-
HWS75_RS08340	1778387..1778833	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
HWS75_RS08345	1778868..1780157	+	1290	WP_003407781.1	threonine ammonia-lyase	-
HWS75_RS08350	1780210..1780413	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HWS75_RS08355	1780419..1780823	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
HWS75_RS08360	1780840..1782582	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
HWS75_RS08365	1782575..1784872	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T166346 WP_003407786.1 NZ_CP058236:1780419-1780823 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T166346 NZ_CP074137:c3583205-3582852 [Serratia sp. JSRIV006]
GTGTGGGAGATTAAAACAACGGATACGTTTGACGAGTGGTTCAGCCGGTTGGAGGATACTGATAGAGCTTCTGTGCTGGC
GTCACTCATTGTACTACGTGAAAAAGGTCCTCAGTTGCCCAGACCCTATGCCGACACGGTAAAGGGATCGCGCTACAGCA
ACATGAAGGAACTACGTGTGCAAAGCCGAGGCATACCGATAAGGGCCTTCTTTGCCTTTGATCCACGCCGTCGCGGTATT
TTGCTTTGTGCTGGTAACAAGGCTGGTAATGAGAAGCGTTTTTATGAGGTGATGATCCCGATTGCCGATCGGGAATTTAC
CCAATTTCTGAATACAGCAGAGAACAAGGAGTAG

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT166346 WP_009935474.1 NZ_CP058236:1780210-1780413 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT166346 NZ_CP074137:c3582847-3582545 [Serratia sp. JSRIV006]
ATGGGCAGAACACTGGAACAAATCCTGGCGGAAGAAAAACCCGAGGTAGTGGCCAGCGCTGAGCGCATTGCGGCGGATAC
GCTGCTCAATATTCACCTGGCCGAGCTACGTGAGCGGGTGCAGAAAACCCAGGTTGAAATGGCGCAGGCACTTGGCATCA
AACAACCCACCGTGGCGGGCATGGAAAAACCAGGCCGCGATCTGAAGCTCTCCTCGCTCAAACGCTATATCGAGGCCGCG
GGGGGCAAGCTGCGTCTGGATATAGAGCTGCCAGATGGTTCGCACTATGGGTTTGTTATTTAA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46