Detailed information of TA system TA166341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1389998..1390686
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: HWS75_RS06635
• Protein ID: WP_003406304.1
• Coordinates: 1390255..1390686 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: HWS75_RS06630
• Protein ID: WP_003406302.1
• Coordinates: 1389998..1390258 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS06610	1385575..1386399	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
HWS75_RS06615	1386404..1387585	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
HWS75_RS06620	1387662..1388762	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
HWS75_RS06625	1388933..1389922	+	990	WP_003909381.1	malate dehydrogenase	-
HWS75_RS06630	1389998..1390258	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
HWS75_RS06635	1390255..1390686	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWS75_RS06640	1390709..1392395	-	1687	Protein_1312	PE family protein	-
HWS75_RS06645	1392575..1393435	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
HWS75_RS06650	1393516..1394346	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
HWS75_RS06655	1394403..1394696	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
HWS75_RS06660	1394693..1394962	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T166341 WP_003406304.1 NZ_CP058236:1390255-1390686 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T166341 NZ_CP074137:c1197891-1197601 [Serratia sp. JSRIV006]
GTGTCACAATTAAAATGGTCACCAGCGGCATTAGCTGATGTGCAACGGCTATACCTTTTTTTGGCAGAAAAGGATAGCGA
CACGGCAAAGCGCGCTATCAGCAAGATCCGCCGTAGCGTAAAAATTCTGGCTCTACAACCACAGGTAGGTCGTCCGGTGG
AGGAGATGCCTCACCAATACCGCGAATGGCCTATTGAATTTGGCAGCAGCGGCTACGTGGTACTGTATCACTTTGATGGC
ACAGTCGCCGTGCTCTTGGCGGTGCGCCATCAGCTTGAAGCGGGTTACTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT166341 WP_003406302.1 NZ_CP058236:1389998-1390258 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT166341 NZ_CP074137:c1198157-1197879 [Serratia sp. JSRIV006]
ATGGCTACCGCGACGTCAATTAAAATCGATGAAGAACTTAAGCTACGTGTGCAGCAAATTGCCCTAGCACGCCAGCGTAC
AGCTCATTGGATTATGCGTGAAGCGATCCGGGAATATGTCGAGCGTGAAGAAAAACGCGAAGCGCTACGCAATGATGCAT
TGCGAGCATGGGAAACCTATCAAGCTGATGGTAAACATCTAACCCAGGAAGAAGCCGATAACTGGCTGGCTAATCTGGAA
AAAGGCGATGATACGGAGATCCCGGAGTGTCACAATTAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6