Detailed information of TA system TA166340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1245394..1245962
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HWS75_RS05965
• Protein ID: WP_003909358.1
• Coordinates: 1245588..1245962 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: HWS75_RS05960
• Protein ID: WP_003405863.1
• Coordinates: 1245394..1245591 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS05940	1241435..1242073	-	639	WP_003898730.1	lipid droplet-associated protein	-
HWS75_RS05945	1242163..1243170	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
HWS75_RS05950	1243187..1244170	-	984	WP_003898731.1	hypothetical protein	-
HWS75_RS05955	1244233..1245306	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
HWS75_RS05960	1245394..1245591	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HWS75_RS05965	1245588..1245962	+	375	WP_003909358.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWS75_RS05970	1246165..1246863	+	699	WP_003909359.1	hypothetical protein	-
HWS75_RS05975	1246981..1247166	+	186	WP_003901093.1	hypothetical protein	-
HWS75_RS05980	1247093..1247368	-	276	WP_003405867.1	hypothetical protein	-
HWS75_RS05985	1247611..1247934	+	324	WP_003405871.1	putative quinol monooxygenase	-
HWS75_RS05990	1247949..1248647	-	699	WP_031667523.1	hypothetical protein	-
HWS75_RS05995	1248681..1248890	+	210	WP_003911400.1	hypothetical protein	-
HWS75_RS06000	1248842..1249612	-	771	Protein_1183	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13391.51 Da
• Isoelectric Point: 4.9046

>T166340 WP_003909358.1 NZ_CP058236:1245588-1245962 [Mycobacterium tuberculosis]
VILVDTSVWIEHLCAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T166340 NZ_CP074137:c741152-740829 [Serratia sp. JSRIV006]
ATGGAATACTTTGAGTTCATCGAAACCCCAGTTTTTAGCAGGCAACGCGAAACCCTATTAGACGATGATAGCTTTCGTGA
ACTACAAACCCACCTGTTAAAATATCACTCAGACGGCGATACTATAAGTAAAACAGGCGGTTGTAAAAAAATCCGATGGA
ACCGTCCAGGCATGGGGAAACAAGGTGGTGTAAGAGTTATCTATTACGTTATCACCCGCAGCGGCAAGCTTTACCTACTG
ATTGTTTATCCTAAAAACGCCAAAGACGACTTAACCGAATCCGAGAAAGCGATACTTAAAGCGTTAACGGATAAACTGAA
TTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT166340 WP_003405863.1 NZ_CP058236:1245394-1245591 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT166340 NZ_CP074137:c740786-740496 [Serratia sp. JSRIV006]
GTGGACAAAGAACTTTTTAACGATCTGGTCGAAAGCATGGAACAGATGGTCGCGATTGAAAAAGGCGAAATGCAGCCTGC
TCGTGTCCATCGCCATCATATCCCCAACGTGAAATCACTACGCGAAGCCAGCGGCATGAAGCAGGAAGAGTTTGCGCAGG
CGGTTGGAGTTAGTCCTTCCCTAGTGCAAAGTTGGGAACAAAATCGCCGAATACCCTCCGGCTCTTCACTCAAAATCTTG
CTGATGATTGAGCGCCACCCCACAATTGTTAACGATCTACTCACCATCTAA

Structures

Antitoxin

• AlphaFold DB: G0TH07