Detailed information of TA system TA166337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1027824..1028797
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HWS75_RS04890
• Protein ID: WP_003909316.1
• Coordinates: 1028297..1028797 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: G0TFR4
• Locus tag: HWS75_RS04885
• Protein ID: WP_003404754.1
• Coordinates: 1027824..1028300 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS04865	1023671..1024942	-	1272	WP_003909315.1	PPE family protein	-
HWS75_RS04870	1024957..1025256	-	300	WP_003404748.1	PE family protein	-
HWS75_RS04875	1025700..1027481	+	1782	WP_003404749.1	BCCT family transporter	-
HWS75_RS04880	1027481..1027735	+	255	WP_003898646.1	hypothetical protein	-
HWS75_RS04885	1027824..1028300	+	477	WP_003404754.1	DUF1778 domain-containing protein	Antitoxin
HWS75_RS04890	1028297..1028797	+	501	WP_003909316.1	GNAT family N-acetyltransferase	Toxin
HWS75_RS04900	1029110..1030429	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
HWS75_RS04905	1030717..1031298	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
HWS75_RS04910	1031298..1032950	+	1653	WP_003404761.1	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18032.50 Da
• Isoelectric Point: 7.8659

>T166337 WP_003909316.1 NZ_CP058236:1028297-1028797 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPPHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T166337 NZ_CP074137:554473-554751 [Serratia sp. JSRIV006]
ATGGAAATTGTTTGGACGCAATTAGCCAGCGAGGACAGGCGCAAAATCCGGGAGTACATTGCTGAAAACAACCTGCGTGC
TGCCATTGAGCTAGACGAGCGGATCGGCTCAATGGCAAACAGCCTGGCCGAGCATCCGTTGAAGGGGCGTGAGGGCCGTG
TTAAAGAAACAAGAGAACTGGTTATCCATCCTCACTTCATTGAGGTTTATCGTGTCGTCAATGAATGGGAGAGGATTGAG
ATCATCCGCGTACTTCATACCGCGCAGCAATGGCCTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT166337 WP_003404754.1 NZ_CP058236:1027824-1028300 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT166337 NZ_CP074137:554204-554473 [Serratia sp. JSRIV006]
ATGACACAGACAACTCTGAATTTACGCATCGACACCGAACTGAAAACTGACTTTTTGGAGGCAGCAAAAGCGATGGATCG
TAATGGTTCGCAGCTCATTCGTGACTTCATGCGTGAGACGGTTCAGCGCCAACATGATATATGGTTCCGTGATCAGGTAC
AGGCTGGAATTGCCCAGGCAAATCGTGGCGAAGTTCTTTCAGGAGAGTCCGTCGAAGCCTCTGCAGTAGCCTGGCGTGAG
GCGGCAGCGAAGAAACTGGCGGGTAGCTGA

Structures

Antitoxin

• AlphaFold DB: G0TFR4