Detailed information of TA system TA166336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1018294..1018913
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: HWS75_RS04835
• Protein ID: WP_003404726.1
• Coordinates: 1018479..1018913 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: HWS75_RS04830
• Protein ID: WP_003404724.1
• Coordinates: 1018294..1018473 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS04815	1013767..1015347	+	1581	WP_003909313.1	serine hydrolase	-
HWS75_RS04820	1015344..1017737	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
HWS75_RS04825	1018010..1018168	+	159	WP_003404720.1	hypothetical protein	-
HWS75_RS04830	1018294..1018473	+	180	WP_003404724.1	antitoxin	Antitoxin
HWS75_RS04835	1018479..1018913	+	435	WP_003404726.1	SRPBCC family protein	Toxin
HWS75_RS04840	1019011..1019784	+	774	WP_003404735.1	VOC family protein	-
HWS75_RS04845	1019849..1020298	+	450	WP_003404738.1	hypothetical protein	-
HWS75_RS04850	1020433..1020663	-	231	WP_003898642.1	hypothetical protein	-
HWS75_RS04855	1020830..1022338	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
HWS75_RS04860	1022340..1023578	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T166336 WP_003404726.1 NZ_CP058236:1018479-1018913 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T166336 NZ_CP074137:c455712-455437 [Serratia sp. JSRIV006]
ATGCTAACTGTCAAATGGACTGACGAAGCCAGAAACGAACTTTACGCACTGATCAGATTTATCGCTGAACAAAATCCGTT
TGCTGCTGAATCACTAATGCAACGGATTGAAGACTCAGTACTTCCTGCTACAGAACACCCCTATCTTTTCCGCCCTGGAC
GTATTCCTGGAACCCGAGAAATTGTGGCGCACCCAAACTATATTGTCGTGTATCAGGTTAAAGATGATTTCCTTGAAGTT
CTTGATGTCCTACATGCTCGCCAGCAATACCCATAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT166336 WP_003404724.1 NZ_CP058236:1018294-1018473 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT166336 NZ_CP074137:c455903-455712 [Serratia sp. JSRIV006]
ATGACGATAAAACTCTCCCCCATCGTCTCCGAGTTTGAAACTCAGGAACAGGCCGACAGTTACGATCGCTGGTTCCGGGC
TAAAGTCGAAGCGGCCATGAACAGCACCAAACCTCGAATCCCCCATGATGAAGTGATGGCTGGTGCACGAAAAATCATTG
AGGAGGCTAAAGCGAGGAGGAAGCAGGCCTGA

Structures

Antitoxin

• AlphaFold DB: G0TFQ5