Detailed information of TA system TA166323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 644499..645175
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: HWS75_RS02915
• Protein ID: WP_003898500.1
• Coordinates: 644499..644912 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: HWS75_RS02920
• Protein ID: WP_003402915.1
• Coordinates: 644909..645175 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS02885	639844..640146	-	303	WP_003909241.1	DUF3349 domain-containing protein	-
HWS75_RS02890	640206..640484	-	279	WP_003402901.1	hypothetical protein	-
HWS75_RS02895	640481..641734	-	1254	WP_003402904.1	inorganic phosphate transporter	-
HWS75_RS02900	641854..642240	-	387	WP_003402907.1	VOC family protein	-
HWS75_RS02905	642303..643187	-	885	WP_003402909.1	SDR family oxidoreductase	-
HWS75_RS02910	643283..644185	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
HWS75_RS02915	644499..644912	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWS75_RS02920	644909..645175	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HWS75_RS02925	645367..647082	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
HWS75_RS02930	647160..648764	+	1605	WP_003402920.1	amidohydrolase	-
HWS75_RS02935	648761..649741	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T166323 WP_003898500.1 NZ_CP058236:c644912-644499 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T166323 NZ_CP074120:4130145-4130480 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT166323 WP_003402915.1 NZ_CP058236:c645175-644909 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT166323 NZ_CP074120:4129897-4130145 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0