Detailed information of TA system TA166312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1986..2533
• Replicon: plasmid pKPCZA02_4
• Accession: NZ_CP058230
• Organism: Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: HWQ20_RS27660
• Protein ID: WP_001384452.1
• Coordinates: 2255..2533 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: HWQ20_RS27655
• Protein ID: WP_215824898.1
• Coordinates: 1986..2255 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ20_RS27650 (46)	46..1077	+	1032	WP_000907874.1	plasmid replication initiator RepA	-
HWQ20_RS27655 (1986)	1986..2255	+	270	WP_215824898.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
HWQ20_RS27660 (2255)	2255..2533	+	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
HWQ20_RS27665 (2573)	2573..3421	+	849	WP_001057996.1	3'-5' exonuclease	-
HWQ20_RS27670 (3538)	3538..4011	+	474	WP_001334658.1	hypothetical protein	-
HWQ20_RS28440 (4488)	4488..4754	-	267	Protein_5	hypothetical protein	-
HWQ20_RS27675 (4754)	4754..5248	-	495	WP_023278058.1	hypothetical protein	-
HWQ20_RS27680 (5304)	5304..5906	-	603	WP_000517689.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-33	-	1..93354	93354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T166312 WP_001384452.1 NZ_CP058230:2255-2533 [Klebsiella pneumoniae]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T166312 NZ_CP074120:c1990915-1990517 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10176.48 Da
• Isoelectric Point: 6.7263

>AT166312 WP_215824898.1 NZ_CP058230:1986-2255 [Klebsiella pneumoniae]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDEVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT166312 NZ_CP074120:c1991211-1990918 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CL36